光谱学与光谱分析, 2010, 30 (5): 1202, 网络出版: 2011-01-26   

灵芝红外光谱的特征峰研究

Study on the Characteristic Peaks of Infrared Spectra from Ganoderma Lucidums
作者单位
浙江工业大学生物与环境工程学院食品系, 浙江 杭州310032
摘要
利用红外光谱法及双指标序列法对不同产地和不同品种灵芝进行红外光谱的特征峰研究, 结果表明: 不同产地和不同品种的绝大多数灵芝红外光谱具有相似的特征峰, 其共有峰率可达到91.67%以上, 但菌丝体品种其共有峰率达到52.94%~58.82%之间, 变异峰率可达29.41%~66.67%之间,主要变异峰在指纹图谱区域1 460.9~1 423.7 cm-1之间出现阶梯峰。 灵芝红外光谱明显的特征峰主要显示为多糖的特征峰型并伴有蛋白质谱带。 在3 377.8~3 396.5 cm-1处有明显的宽而强的吸收峰, 在2 924.2~2 925.1 cm-1有小肩峰, 在1 635.8~1 650.3 cm-1处有中等强度的吸收峰, 在1 372.5~1 375.2 cm-1处有中等强度的吸收峰, 在1 074.8~1 075.3和1 043.2~1 045.2 cm-1处有很强的吸收分叉尖峰, 在指纹图谱区域891.0~894.8 cm-1处有明显的弱峰。 在指纹图谱区域563.10~574.7 cm-1有中等强度的吸收峰。
Abstract
Infrared spectroscopy and dual-indicator sequence were used to study their IR characteristic peaks of ganoderma lucidums from different places and species. The experimental results showed that the majority of ganoderma lucidum from different places and species had the similar characteristic peaks of infrared spectra, Their common peak ratio could reach more than 91.67%, but the common peak ratio of mycelium was 52.94%-58.82%, and variant peak ratio was 29.41%-66.67%. The main variation peak was in the fingerprint regions 1 460.9-1 423.7 cm-1with ladder peaks. The IR characteristic peaks of ganoderma Lucidum obviously appear as the main characteristic peaks with the type of polysaccharide and protein bands. There was obviously a wide and strong absorption peak in 3 377.8-3 396.5 cm-1, a small acromion in 2 924.2-2 925.1 cm-1, a medium intensity absorption peak in 1 635.8-1 650.3 and 1 372.5-1 375.2 cm-1, a strong absorption bifurcate peak in 1 074.8-1 075.3 and 1 043.2-1 045.2 cm-1, a obvious weak peak in fingerprint regions 891.0-894.8 cm-1, and a medium intensity absorption peak in 563.10-574.7 cm-1.

何晋浙, 邵平, 孙培龙. 灵芝红外光谱的特征峰研究[J]. 光谱学与光谱分析, 2010, 30(5): 1202. HE Jin-zhe, SHAO Ping, SUN Pei-long. Study on the Characteristic Peaks of Infrared Spectra from Ganoderma Lucidums[J]. Spectroscopy and Spectral Analysis, 2010, 30(5): 1202.

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