尝试利用漫反射光谱检测鲜红斑痣皮肤的氧含量, 并用于监测光动力治疗中鲜红斑痣皮肤氧含量的变化. 采用Ocean Optics公司生产的USB2000微型光纤光谱仪采集PWS皮肤的漫反射光谱, 根据氧合血红蛋白与还原血红蛋白(皮肤中两种主要的吸光基团)吸收光谱的差异, 分析病变皮肤氧含量及治疗中氧含量的变化趋势. 对14例PWS患者进行治疗中皮肤漫反射光谱的监测. 结果显示, 治疗前不同类型PWS皮肤氧含量不同, 光动力治疗中大部分P4、 P5型PWS皮肤氧含量没有显著降低, P6型PWS皮肤氧含量降低明显. 表明现有的漫反射光谱法可定性监测PWS皮肤氧含量的变化趋势. 通过进一步提高仪器的灵敏度并建立相应的光谱分析方法, 有望更准确地测量PWS皮肤中的氧含量变化.

To measure oxygen content in port wine stain (PWS) skin using diffuse reflectance spectroscopy and monitor the change in oxygen content during photodynamic therapy (PDT), the diffuse reflectance spectra of port wine stain skin were measured using the USB2000 miniature fiber optic spectrometer immediately 10, 20, 30 and 40 min after treatment began, then oxygen content at each time point was analyzed based on the difference of absorption spectra between Hb and HbO2, and the change trend can be observed. The diffuse reflectance spectra of 14 PWS patients were monitored during treatment, and the change trend of oxygen content were analyzed. Results showed that before treatment the oxygen content varies between patients with different type of PWS. During PDT, there was no obvious change in oxygen content for skin of most P4 and P5 PWS, while obvious decline was observed in oxygen content for P6 PWS. The current diffuse reflectance spectroscopy can be used to monitor oxygen content in PWS skin qualitatively and observe the change trend. To get more precise oxygen content in PWS skin, more work is needed to improve the sensitivity of diffuse reflectance spectroscopy and a spectra algorithm should be established.