用β-环糊精作为手性选择试剂, 利用甲霜灵中R和S异构体与β-环糊精相互作用强弱不同而导致的紫外-可见光谱的差异来检测甲霜灵手性异构体的比例, 偏最小二乘法建立甲霜灵手性异构体比例的定量模型, 并对模型效果进行评价. 甲霜灵-环糊精体系中甲霜灵R-异构体比例的定量模型校正集的决定系数R2为0.999 0, 校正集标准偏差SEC为0.006 7, 相对标准偏差RSD为0.89%; 外部检验集6个样品的预测值与化学值的相关系数为0.998 5, 外部检验集标准差SEP为0.008 9, 相对标准偏差RSD为1.17%, 本方法简单快速, 具有重要的实际应用价值.

In the present study, β-cyclodextrin(β-CD) was used as chiral selector to detect the proportion of chiral isomers of metalaxyl. The proportion of metalaxyl enantiomers can be detected by ultraviolet(UV)spectroscopy since the interaction between the R, S isomer of metalaxyl with β-CD is different. The quantitative models were established by partial least squares regression (PLS) and the robust of models was evaluated by independent validation samples. The determination coefficient R2 of calibration set in the quantitative model was 0.999 0. The standard error of calibration set (SEC) and the relative standard deviation (RSD) of the model was respectively 0.006 7 and 0.89%; The correlation coefficient r of estimated value and specified value of the 6 independent validation samples was 0.998 5. The standard error of prediction (SEP) and RSD was respectively 0.008 9 and 1.17%. This method is rapid and easy to operate in practical applications.