Chinese Optics Letters, 2011, 9 (2): 022801, Published Online: Mar. 3, 2011
Immunosensor application based on Raman spectroscopy of porous silicon Download: 558次
多孔硅 免疫传感器 猪丹毒丝菌表面保护性抗原A 拉曼光谱 光学检测 280.1415 Biological sensing and sensors 070.0070 Fourier optics and signal processing 070.4790 Spectrum analysis 280.0280 Remote sensing and sensors
Abstract
Porous silicon (PS) suitable for optical detection of immunoreaction is fabricated. The structure of immunosensor is prepared by the following steps: oxidization, silanization, glutaraldehyde cross-linker, and covalent binding of antibody. When antigen is added into the immunosensor, the Raman intensity is estimated to be linearly reduced according to the concentration of the surface protective antigen protein A (spaA) of below 4.0 μg·ml?1. The ultimate detection limit is 1.412×10^2 pg.ml^{?1}. Controlled experiments are also presented with non-immune antigen of the spaA, and results show that the immunosensor has high specificity. Compared with the conventional enzyme-linked immuno sorbent assay (ELISA), this method is quick, inexpensive, and label-free.
Tao Jiang, Jiaqing Mo, Xiaoyi Lv, Pengfei Yan, Zhenhong Jia, Jiangwei Li, Fuchun Zhang. Immunosensor application based on Raman spectroscopy of porous silicon[J]. Chinese Optics Letters, 2011, 9(2): 022801.