Non-invasive monitoring of living cell culture by lensless digital holography imaging
A non-invasive detection method for the status analysis of cell culture is presented based on digital holography technology. Lensless Fourier transform digital holography (LFTDH) configuration is developed for living cell imaging without prestaining. Complex amplitude information is reconstructed by a single inverse fast Fourier transform, and the phase aberration is corrected through the two-step phase subtraction method. The image segmentation is then applied to the automatic evaluation of confluency. Finally, the cervical cancer cell TZMbl is employed for experimental validation, and the results demonstrate that LFTDH imaging with the corresponding image post-processing can provide an automatic and non-invasive approach for monitoring living cell culture.
王大勇：College of Applied Sciences, Beijing University of Technology, Beijing 100124, China
赵洁：College of Applied Sciences, Beijing University of Technology, Beijing 100124, China
杨怡殊：College of Life Science and Biotechnology, Beijing University of Technology, Beijing 100124, China
肖向茜：College of Life Science and Biotechnology, Beijing University of Technology, Beijing 100124, China
崔华坤：College of Applied Sciences, Beijing University of Technology, Beijing 100124, China
备注：This work was supported by the National Natural Science Foundation of China (No. 61077004), the Science Foundation of Education Commission of Beijing (No. KZ200910005001), and the Innovative Talent and Team Development for Serving Beijing.
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Yunxin Wang, Dayong Wang, Jie Zhao, Yishu Yang, Xiangqian Xiao, Huakun Cui, "Non-invasive monitoring of living cell culture by lensless digital holography imaging," Chinese Optics Letters 9(3), 030901 (2011)