采用旋涂法制备了若丹明6G（Rh6G）和若丹明B（RhB）两种染料掺杂的DNA-CTMA复合薄膜，并通过对薄膜的表面形貌、紫外/可见/红外吸收光谱、荧光光谱特性的测量，表征了薄膜的成膜质量及光谱特性。通过NdYAG脉冲激光器的倍频光激发，研究了不同比例荧光染料掺杂DNA-CTMA薄膜的放大自发辐射（ASE）现象并做了理论解释。实验结果表明，在波长为532 nm的脉冲激光抽运下，Rh6G/RhB/DNA-CTMA薄膜的发射谱出现了明显的窄化现象，而且比单一染料掺杂DNA-CTMA薄膜的发射谱呈现出较大的光谱调谐范围，是一种较好的有机聚合物激光材料体系。

The fluorescent dye Rhodamine-6G (Rh6G) and Rhodamine-B (RhB) doped DNA-CTMA thin films are prepared by spin-coating technology. The quality and optical spectra properties of Rh6G/RhB/DNA-CTMA thin films are characterized by the measurements of surface morphology, UV/vis/infrared absorption spectra and fluorescence spectra. The amplified spontaneous emission (ASE) of dye-doped DNA-CTMA thin films with different dye contents was researched experimentally by pumping of NdYAG pulse laser at wavelength of 532 nm, and is explained theoretically. The experimental results show that the narrowing phenomenon of emission spectra of Rh6G/RhB/DNA-CTMA thin films is very clear and strong. Compared with single dye doped DNA-CTMA thin films, the co-doped Rh6G/RhB/DNA-CTMA thin film is a potential candidate as an organic polymer laser material because it exhibits more wider tuning range of the ASE spectrum.