运用FTIR技术, 结合外标法、 二阶导数和曲线拟合方程, 研究曼地亚和南方红豆杉红外光谱特征及其不同部位间紫杉醇含量的差异。 结果显示: (1)向原药材中添加紫杉醇量≥0.004 5 mg时, 在1 516, 1 371, 1 244, 1 109和773 cm-1等处的吸收峰明显增强, 且二阶导数图谱 “指纹”区上述等处的峰强随添加紫杉醇浓度的增加而升高; (2)对1 058宽峰运用Gaussian函数曲线拟合发现, 曼地亚红豆杉拟合出8个子峰而南方红豆杉拟合出7个子峰; (3)二阶导数和曲线拟合方程分析表明, 两种红豆杉紫杉及其不同部位紫杉醇含量均存在一定差异, 具体而言, 曼地亚红豆杉超过南方红豆杉; 不同部位比较时, 曼地亚红豆杉以叶片中含量最高而南方红豆杉根中含量最高。 所以, 上述方法, 可以快速分析评价两种红豆杉及其不同部位紫杉醇含量的差异。

In the present study, combined with external standard method, second derivative as well as curve-fitting equation, the infrared spectroscopy techniques were applied to research the discrepancy of paclitaxel content among different parts of them repectively as well as the differences of infrared spectral character between Taxus Media(T. Media) and Taxus Mairei (T. Mairei). The results showed: (1) The band around 1 516, 1 371, 1 244, 1 109 and 773 cm-1 was markedly enhanced when paclitaxel standard sample was added by more than 0.004 5 mg to original traditional Chinese materials, in addition, in infrared fingerprint area, the second derivative spectra show that there was good corresponding peak between traditional Chinese materials and standard paclitaxel sample around 1 371, 1 315, 1 244, 1 143, 1 106, 1 070, 1 022 and 773 cm-1, furthermore, the intensity of above character band would increase accompanying with increased standard paclitaxel sample. So, the band character around 1 371, 1 315, 1 244, 1 143, 1 106, 1 070, 1 022 and 773 cm-1 could be used to evaluate paclitaxel content of T. media and T. mairei; (2) Around 1 800～700 cm-1, IR spectral features suggest that two kinds of Chinese yew had quite similar infrared vibration character, but when Gaussian function was applied to decompose the band around 1 058 cm-1, the result demonstrated that the T. media were decomposed with 8 while T. mairei were only decomposed with 7 component bands. (3) Second-derivative and curve-fitting equation analysis demonstrated that there were certain differences of paclitaxel content between T.media and T.mairei as well as different parts of them. Specifically, the paclitaxel content of T. media was higher than T. mairei, while the paclitaxel content in leaf of T. media was highest, on the contrary, the paclitaxel content in root of T. mairei was highest when comparing the content among the different parts of T. media and T. mairei respectively. Therefore, above methods could be quickly analyze and evaluate the differences of paclitaxel content between T. media and T. mairei as well as the different parts of them.