用异硫氰酸荧光素作为荧光探针, 在碱性条件下标记酪蛋白, 根据标记前后吸收光谱、 荧光光谱的变化情况对异硫氰酸荧光素和酪蛋白相互作用进行了初步探讨。 用SephadexG-50层析柱分离出荧光标记物, 以荧光标记酪蛋白作乳化剂, 采用喷雾干燥法制备荧光标记酥油微胶囊, 用激光扫描共聚焦显微镜在488 nm的Ar+激光光源激发下断层扫描酥油微胶囊微结构。 结果表明, 酪蛋白是在油水界面膜和微胶囊表面聚结。 制备出的酥油微胶囊有单核和多核两种形式, 微胶囊为园球形, 表面光滑, 无裂缝, 无凹陷, 微胶囊壁表面完整, 壁结构较为致密, 其颗粒尺寸为有明显差异的大小颗粒组成, 而且小颗粒附着在大颗粒上, 形成了部分附聚粉, 有助于微胶囊溶解, 是一种较为理想的微胶囊制品。

In the present paper, fluorescein isothiocyanate was chosen as a fluorescence probe to mark casein protein in alkaline conditions. The interaction of the casein protein marked or not marked and fluorescein isothiocyanate was preliminarily discussed by the spectrum changes of UV-absorption and fluorescence spectrometry. Fluorescent marker was separated from SephadexG-50 chromatography column. With it as an emulsifier, the fluorescently-labeled ghee microcapsules were prepared by spray drying. And using laser scanning confocal microscope by tomoscan imaging to detect the microstructure of ghee microcapsules with the excitation of 488 nm argon-ion laser, the results showed that the casein protein assembled in the membrane surface of oil-water interface and microcapsules. The ghee microcapsules had two forms, namely mononuclear and multinuclear. The microcapsule was spherical. Its surface was smooth with no crack and no hollow. Its wall surface was intact and wall structure was relatively dense. The particle size showed obvious difference. Small particles attached to large particles, forming partial agglomerating powders to contribute to enhancing the solubility of microcapsules. These prove that the ghee microcapsule is an ideal microcapsule product.