光学学报, 2011, 31 (6): 0630001, 网络出版: 2011-05-18   

基于新型NIR-SERS基底的肝癌血清NIR-SERS光谱研究

Investigations on NIR-SERS Spectra of Serum for Liver Cancer Based on NIR-SERS Substrate
作者单位
1 楚雄师范学院光谱技术应用研究所, 云南 楚雄 675000
2 楚雄彝族自治州人民医院, 云南 楚雄 675000
3 云南师范大学物理与电子科学系, 云南 昆明 650092
摘要
基于一种新型、高效、生物兼容性近红外表面增强拉曼散射(NIR-SERS)基底,采用便携式近红外拉曼光谱仪分别对健康人和肝癌病患者的血清进行了NIR-SERS光谱研究。实验发现,健康人与肝癌患者的血清NIR-SERS光谱存在显著差异:1)健康人血清NIR-SERS 光谱中位于630、720、812和1578 cm-1附近的谱峰在肝癌患者血清NIR-SERS 光谱中变得很弱,甚至消失;2)健康人血清NIR-SERS光谱中位于1130 cm-1和1204 cm-1附近的谱峰在肝癌患者血清NIR-SERS 光谱中分别蓝移到1135 cm-1和1269 cm-1附近,同时在558 cm-1附近出现一新的较强拉曼峰;3)对健康人和肝癌患者血清NIR-SERS光谱中位于300、630、1130和1578 cm-1等处的拉曼峰相对峰强度比研究发现,相对峰强度比I630/I300,I1130/I300和I1578/I300可以作为较好的区分健康人和肝癌患者的NIR-SERS光谱诊断指标,这为基于SERS光谱技术进行肝癌光谱诊断提供了初步的实验依据。
Abstract
Investigations on near infrared surface-enhanced Raman scattering (NIR-SERS) spectra of the serum for patients with liver cancer and healthy persons based on Ag nanofilms prepared by using electrostatic self-assembly are reported. Analysis indicates NIR-SERS spectra of the sera between healthy persons and liver cancer patients are different. Firstly, Raman bands at 630, 720, 812 and 1578 cm-1 become weaker (even disappear) in NIR-SERS spectra of the sera for cancer liver patients but stronger in NIR-SERS spectra of the sera for healthy persons. Secondly, Raman bands at 1130 cm-1 and 1204 cm-1 in NIR-SERS spectra of the sera for healthy persons have blue shifts to 1135 cm-1 and 1269 cm-1 in NIR-SERS spectra of sera for cancer liver patients, respectively. Meanwhile, a new Raman band at 558 cm-1 appears in NIR-SERS spectra of liver cancer patients. Additionally, striking spectral differences are abvious in NIR-SERS spectra in the intensity ratios at 630/300, 1130/300, and 1578/300 cm-1. These three peak-intensity ratios of liver cancer patients with values of 0.848±0.042, 1.094±0.118, 0.914±0.070, respectively, are more notable (mean±S.D., n=15, P<0.01) compared with those of healthy persons (1.985±0.487, 1.568±0.286, 1.189±0.108, respectively). The results show that the intensity relative peak-ratios at 630/300, 1130/300, and 1578/300 cm-1 can be used to discriminate liver cancer patients from healthy persons, which indicate these three intensity ratios can be served as NIR-SERS spectral criteria for the diagnosis of cancer liver.

刘仁明, 刘瑞明, 武延春, 柳振全, 张德清, 自兴发, 司民真. 基于新型NIR-SERS基底的肝癌血清NIR-SERS光谱研究[J]. 光学学报, 2011, 31(6): 0630001. Liu Renming, Liu Ruiming, Wu Yanchun, Liu Zhenquan, Zhang Deqing, Zi Xingfa, Si Minzhen. Investigations on NIR-SERS Spectra of Serum for Liver Cancer Based on NIR-SERS Substrate[J]. Acta Optica Sinica, 2011, 31(6): 0630001.

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