以灰黄霉素产生菌 GM120-43为出发菌株, 采用半导体激光和 CO2激光进行诱变。结果, 仅在 100 mW低功率条件下, 半导体激光诱变获得正突变菌株 LD100-1, 突变株 LD100-1固体发酵产灰黄霉素的效价为 119 766 u/g, 与出发菌株 GM120-43相比提高了 33.82%, 且遗传性状稳定; 用 CO2激光进行诱变时, 获得突变株 C1448-1, 其固体发酵产灰黄霉素比原始菌株提高22.58%, 但是性状不稳定, 在传代过程中可能发生回复突变, 效价降低于出发菌株。

The Griseofulvin producing strain GM120-43 was taken as the original strain, which was mutated by LD-Laser and CO2 Laser. The results indicated that the mutant strain LD100-1 was obtained just at 100 mW low power when mutated by LD-Laser. The griseofulvin produced by LD100 -1 with solid -state fermentation, which titer of was 119 766 u/g and it was 33.82% higher than original strain GM120-43,the genetic stability of the high productivity was stable at the same time. The mutant strain C1448-1 was obtained by CO2 Laser, which solid-state fermentation producing griseofulvin was 22.58% higher than parent strain, but the character of mutant strain C1448-1 was not stable, which may happened reversible mutation that cause the titer of this strain was lower than original strain.