研究了以铜(Ⅱ)离子作为模板离子, 合成了铜(Ⅱ)离子印迹聚合物并将其做成固相萃取柱, 直接安装在流动注射系统上, 对样品中的铜(Ⅱ)离子进行在线分离富集; 经H2SO4+乙醇混合溶液洗脱液在线洗脱后。 在H2SO4介质中, 铜(Ⅱ)离子催化高锰酸钾氧化桑色素发生化学发光反应。 据此建立了灵敏简单流动注射化学发光法测定铜的新方法。 在最佳实验条件下, 测定铜的线性范围为0 .1～10 μg·mL-1, 相关系数r=0.996 8, 方法检出限为0.001 2 μg·mL-1, 对4.0 μg·mL-1铜进行9次测定的相对标准偏差小于3%, 加标回收率在95%～105%之间, 方法用于样品中铜的测定, 结果满意。

In the present study, Cu(Ⅱ)-imprinted polymer was prepared and used as an effective method for separation and enrichment of Cu(Ⅱ) ion in samples. An on-line preconcentration and extraction flow-injection system using solid phase extraction (SPE) column packed with prepared ion imprinted polymer (IIP) was set up. The column was eluted with a mixture of ethanol and H2SO4 after preconcentration. A sensitive and simple chemiluminescence(CL) method using flow-injection has been developed for the determination of copper. The method is based on the fact that traces of Cu(Ⅱ) exert a catalytic action on the oxidation between morin by KMnO4 solution. After optimizing the different experimental parameters, a calibration graph was obtained over the concentration range of 0 to 10 μg·mL-1 for Cu(Ⅱ) and r=0.996 8. The detection limit is 0.001 2 μg·mL-1. The recoveries are between 95% and 105% and the standard relative deviations( RSD) are less than 3% at 4.0 μg·mL-1 (n=9) level was obtained. The new chemiluminescence determination for copper has been established. The method can be satisfactorily applied to the determination of copper in sample.