为了提高皮肤荧光光谱的收集效率，对用于皮肤组织荧光光谱检测的光纤探头设计进行了重点研究。通过建立人体皮肤组织光学模型，并利用Monte Carlo方法模拟光在光纤探头及皮肤组织中的传播过程，研究光纤数量、光纤芯径、数值孔径、入射光纤与收集光纤近边缘距离、光纤探头与皮肤的距离等光纤探头各特性参数对荧光光谱检测的影响；设计、制作了一种光纤探头，应用于人体皮肤晚期糖基化终末(AGE)产物荧光光谱检测中，分析检测效果，并进行模拟与实验的对比研究。结果表明，较之于常规光纤探头，根据Monte Carlo模拟进行设计的光纤探头，荧光光谱检测性能有明显改进，且模拟与实验结果有一致的变化趋势，验证了通过Monte Carlo模拟进行光纤探头设计的可行性。光纤探头特性参数对皮肤荧光光谱检测有着显著影响，针对不同检测目的，设计特定的光纤探头，能够改善基于荧光光谱检测的医疗设备的性能。

Fiber-optic probe design is studied to improve the efficacy of collection of tissue fluorescence. After optical models of human skin tissue is developed, Monte Carlo method is employed to simulate light propagation in fiber-optic probe and skin tissue, and to investigate the effect of fiber-optic probe parameters, including number of fibers, fiber diameter, numerical aperture, source-collection fiber separation distance, fiber-tissue spacer thickness, on light propagation and fluorescence collection. Then, a fiber-optic probe is designed to detect fluorescence of human skin advanced glycation end (AGE) products, the performance of the probe is analysed, and comparison between simulation and experiment was carried out. Results indicate that, compared with a common fiber-optic probe, the designed probe has better ability to collect fluorescence and decrease reflected light of skin tissue. In addition, the consistency of experiment and simulation result indicates that Monte Carlo method can be used in the design of fiber-optic probe. Fiber-optic probe parameters can affect light propagation and fluorescence detection obviously. Designing different fiber-optic probes according to different application purposes, can improve its efficacy of fluorescence based diagnostic devices.