建立了大鼠骨缺损模型，评价了低能量激光治疗处理后骨缺损处的组织学改变。制造大鼠单侧的股骨圆形骨缺损，根据低能量激光治疗照射方法的不同随机分为3组：多次照射组，单次照射组，对照组。其中两个照射组对应的总剂量相同。术后15 d和30 d分别处死每组动物，取材后进行HE染色，在光镜下分析炎症和骨组织的形成和质量。结果显示实验组15 d和30 d时的炎症评分明显低于对照组，骨形成评分明显高于对照组，而2个实验组并无明显区别。研究发现适宜剂量的低能量激光治疗可以减轻动物模型实验性骨缺损处的炎症并促进新骨形成。多次照射和单次照射比较并无不同。

The histological changes of bone defect sites created surgically in rat femur treated by low level laser therapy (LLLT) are evaluated. Thirty rats are randomly divided into 3 groups according to different methods of laser irradiation: multi-irradiated group, single-irradiated group and control group with no irradiation. The total doses of both of the irradiation groups are the same. A round bone defect is created on the femur of each rat. The rats are killed on the 15th day and the 30th day after the surgery. The results show that after drawing materials and HE staining, the inflammation and bone formation of each group are analyzed under an optical microscope. The inflammation scores of irradiated groups are lower than those of control group with significant difference, meanwhile, the bone formation scores of irradiated groups are higher than those of control group with significant difference. However, there is no significant difference between the two irradiation groups. It is concluded from the study that LLLT with proper dose may alleviate inflammation and promote bone formation. There is no difference between multi-irradiated group and single-irradiated group.