采用傅里叶变换红外光谱结合二阶导数谱和二维相关红外光谱的三级鉴定技术对肉苁蓉的不同浓度醇提物药液和相应的药渣由表及里地进行了分析与评价研究。 结果表明， 用乙醇提取肉苁蓉活性成分的加工过程使苯乙醇苷类等活性物质在红外光谱上得以明显体现， 并发现70％乙醇提取物中苯乙醇苷类等物质均高于其他浓度提取物。 该技术从整体上揭示了乙醇萃取肉苁蓉活性物质的规律， 验证了传统醇提法的合理性。 它不仅可以用于跟踪肉苁蓉醇提取过程和药液药渣的成分分析， 分辨不同浓度提取液和药渣成分的细微差别， 还可以将不同浓度提取液和药渣成分相结合， 提供肉苁蓉药材的整体信息， 为更深入的肉苁蓉的成分分析和药效研究提供宏观整体上的指导。

Tri-step identification of Fourier transform infrared spectroscopy (FTIR) integrated with second derivative spectroscopy and two dimensional correlation infrared spectroscopy (2D-IR) were applied to analyze and evaluate the alcoholic extracts and corresponding residues of cistanche deserticola from the surface to what lies behind. It was found that active compounds including phenylethanoid glycosides were enriched effectively after alcoholic extraction and the extract by using 70% of alcohol had the highest concentration compared to the others. The technique of the tri-step identification holistically disclosed the profile of active compounds in cistanche deserticola extracted by a series of concentrations of ethanol and validated the rationality of the traditional alcoholic extraction method. It not only could be used in monitoring the process of the alcoholic extraction and the compositions of the extracts and residues, discriminating micro-differences among them, but also could provide a macroscopic guidance for medicinal and pharmacological studies.