光电工程, 2012, 39 (1): 140, 网络出版: 2012-02-13  

成像检测在 DNA毛细管电泳中的应用

Imaging Detection Applied in DNA Capillary Electrophoresis
作者单位
上海理工大学光电信息研究院,上海 200093
摘要
本文研究了成像检测方式以及阵列检测器在 DNA毛细管电泳的荧光检测中的应用,目的在于减小检测过程引起的样品区带增宽,提高 DNA毛细管电泳的分辨率和分离速度。依据分离度理论以及模拟计算结果,归纳了检测宽度的影响规律,指出减小检测宽度对于高效分离,特别是高速微流控系统电泳分离的重要性。实验测量并比较了成像检测与传统光强检测的结果。提出阵列检测器的信号增强方法,并进行了模拟验证。研究表明,成像检测可显著减小毛细管电泳的检测宽度,提高分离效率和分离速度。
Abstract
Imaging detection and Charge Coupled Device (CCD) detector was adopted in fluorescence detection of DNA capillary electrophoresis in order to decrease the detection width and improve the efficiency and speed of DNA separation. The importance of small detection width for high-speed capillary electrophoresis, especially the separation performed in micro-fluid device, was illustrated according to theoretical calculation based on resolution theory of capillary electrophoresis. The performances of imaging detection and conventional intensity detection were measured and compared. The method of data processing to intensify the DNA band signal of CCD detector was proposed, and verified by simulation. Imaging detection and its data processing method can remarkably decrease the detection width of capillary electrophoresis, and then improve the efficiency and speed of DNA separation.

陈进, 王伟, 程抒一, 倪一. 成像检测在 DNA毛细管电泳中的应用[J]. 光电工程, 2012, 39(1): 140. CHEN Jin, WANG Wei, CHENG Shu-yi, NI Yi. Imaging Detection Applied in DNA Capillary Electrophoresis[J]. Opto-Electronic Engineering, 2012, 39(1): 140.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!