利用荧光光谱研究了三种异黄酮类化合物染料木素、 鸡豆黄素A和 3’,4’,7-三羟基异黄酮与不同异构体人血清白蛋白的相互作用机制。 计算了异黄酮与蛋白质形成复合物的各种结合参数(猝灭速率常数、 结合常数及结合位点数), 结果表明三种异黄酮类化合物在人血清白蛋白上只有一个结合位点, 位于结合位点site Ⅰ, 结合常数在0.17×105~1.20×105 L·mol-1之间。 荧光增强光谱结果显示, 与蛋白质作用后药物的荧光强度明显增加, 说明了药物与人血清白蛋白发生了结合, 在此基础上讨论了三种异黄酮与人血清白蛋白的结合机理。

The interaction mechanisms between isoflavones (Genistein, 3’,4’,7-trihydroxyisoflavone and Biochanin A) and different isomers of human serum albumin (HSA) were investigated by fluorescence spectroscopy. Various parameters (quenching rate constants, binding constants and number of binding sites) of isoflavones-human serum albumin complexes were calculated. The results showed that the isoflavones have only one binding site on human serum albumin, located at the Site I, and the binding constants were between 0.17×105 and 1.20×105 L·mol-1. Fluorescence enhancement experiments showed that the fluorescence intensities of the drugs significantly increased after interacting with HSA, indicating that the combination of drug and HSA had occurred. The binding mechanisms between three isoflavones and HSA were discussed.