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金颗粒为活性基底的裸鼠血清表面增强拉曼散射光谱分析

Surface Enhanced Raman Scattering Spectrum Analysis of Nude Mouse Serum with Au Nanoparticles Active Substrate

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摘要

对肿瘤裸鼠血清进行显微共聚焦拉曼光谱测量,并将血清的普通拉曼光谱与以金纳米颗粒为基底的表面增强拉曼散射(SERS)光谱进行对比,结果表明金纳米粒子作为活性基底对裸鼠血清具有显著的拉曼增强作用,在很多常规拉曼光谱中未能检测出的信息在SERS光谱中得以体现。这源于血清中的物质与金纳米颗粒之间的化学吸附和相互作用,使得微量样品也能够通过SERS光谱来反映出血清中各种蛋白质、脂类、糖类、核酸以及其他成分的分子结构等信息。血清中各种成分的信息可以反映体内各种细胞和组织的生理代谢及病理变化,血清的SERS光谱将为疾病的诊断和治疗提供一种有效手段。

Abstract

Raman spectrum and surface enhanced Raman scattering (SERS) spectrum of the nude mouse serum are measured using laser Raman confocal micro-spectrometry. The results show that the Au nanoparticles active substrate can enhance the Raman spectra dramatically. A lot of information which conventional Raman spectroscopy fails to detect emerges. This is due to the chemical adsorption and interactions between the gold nanoparticles and the substances in serum. The information is important for the analysis of the most components and structures of the serum proteins. With SERS spectrum, even micro-sample can also reflect the molecular structure information of the serum proteins, lipids, carbohydrates, ribonucleic acid and other components. Information of the serum components can reflect physiological and pathological metabolism changes of the cells and tissues in the body. So SERS spectrum of the serum would provide an effective means for the diagnosis and therapy of diseases.

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中图分类号:TN249

DOI:10.3788/cjl201239.0504004

所属栏目:生物医学光子学与激光医学

责任编辑:韩峰  信息反馈

基金项目:国家自然科学基金(61027015,61177088,61107076)和上海市科委重点项目及重点学科建设项目(10DZ2210900,S30108)资助课题。

收稿日期:2012-01-11

修改稿日期:2012-02-07

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刘书朋:上海大学特种光纤与光接入网省部共建国家重点实验室, 上海 200072
朱鸿飞:上海大学特种光纤与光接入网省部共建国家重点实验室, 上海 200072
陈娜:上海大学特种光纤与光接入网省部共建国家重点实验室, 上海 200072
陈振宜:上海大学特种光纤与光接入网省部共建国家重点实验室, 上海 200072
胡玲:上海大学特种光纤与光接入网省部共建国家重点实验室, 上海 200072

联系人作者:刘书朋(liusp@shu.edu.cn)

备注:刘书朋(1971—),男,博士,副教授,主要从事生物医学工程方面的研究。

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引用该论文

Liu Shupeng,Zhu Hongfei,Chen Na,Chen Zhenyi,Hu Ling. Surface Enhanced Raman Scattering Spectrum Analysis of Nude Mouse Serum with Au Nanoparticles Active Substrate[J]. Chinese Journal of Lasers, 2012, 39(5): 0504004

刘书朋,朱鸿飞,陈娜,陈振宜,胡玲. 金颗粒为活性基底的裸鼠血清表面增强拉曼散射光谱分析[J]. 中国激光, 2012, 39(5): 0504004

被引情况

【1】胡琪,赵进辉,袁海超,洪茜,肖海斌,刘木华. 基于表面增强拉曼光谱的甲萘威水溶液检测分析. 激光与光电子学进展, 2014, 51(7): 73004--1

【2】林居强,阮秋咏,陈冠楠,冯尚源,李步洪,陈荣. 基于表面增强拉曼散射技术的癌症检测研究进展. 激光与光电子学进展, 2013, 50(8): 80020--1

【3】杨必文,郭周义,刘智明,万明明,覃筱楚,钟会清. 绿色合成银纳米粒子及其在SERS中的应用. 光谱学与光谱分析, 2013, 33(7): 1816-1819

【4】董晶晶,陈娟,戈延茹,戚雪勇. 激光拉曼光谱法无损鉴别三七及其伪品. 激光与光电子学进展, 2014, 51(5): 53002--1

【5】李金金,卢明倩,张晶晶,黄庶识,陈丽梅,年洪娟. 拉曼光谱技术研究铝胁迫下的土生隐球酵母细胞凋亡. 中国激光, 2014, 41(11): 1115002--1

【6】熊洋,司民真,高飞,张德清. 基于NIR-SERS光谱技术分析宫颈癌氧合血红蛋白. 中国激光, 2015, 42(1): 115001--1

【7】张川云,司民真,李伦,张德清. 基于顶空-SERS技术的不同产地、不同药源的薤白挥发物研究. 光学学报, 2015, 35(3): 330001--1

【8】马海宽,张旭,钟石磊,史晓凤,马丽珍,马君. 基于静电富集-表面增强拉曼光谱联用技术的抗生素检测. 中国激光, 2018, 45(2): 207028--1

【9】杨玥,翁国军,赵婧,李剑君,朱键,赵军武. 纸质表面增强拉曼散射基底的制备及其应用进展. 中国激光, 2018, 45(3): 307011--1

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