以丹磺酰胺为荧光基团设计合成了新型Zn2+荧光探针DW1(5-(二甲基氨基)-N-(4-(2-(2-喹啉亚甲基)甲酰肼基)苯基)萘-1-磺酰胺)。通过紫外光谱、荧光光谱及电喷雾质谱研究了DW1对Zn2+的选择性识别作用。结果表明, DW1与Zn2+结合后荧光显著增强, 荧光发射光谱由545 nm蓝移至515 nm, 量子产率达到0.32, 且对Zn2+具有较高的选择性, 受常见离子的干扰较小。光谱滴定和ESI-MS谱表明DW1与Zn2+以1∶1的化学计量数形成配合物, 平衡常数K=1.75×104(mol/L)-1。

A novel dansyl sulfonamide-based fluorescence probe, (E)-5-(dimethylamino)-N-(4-(2-(quinolin-2-ylmethylene)hydrazinecarbonyl)phenyl)naphthalene-1-sulfonamide (DW1) was synthesized for detection of Zn2+. UV-Vis, fluorescence and ESI-MS spectra of DW1 were measured to investigate the selective recognition. The results show that the fluorescence intensity increase significantly with high quantum yield (Φf=0.32) and blue-shift emission from 545 nm to 515 nm after the coordination of DW1 with Zn2+, manifesting DW1 could detect Zn2+ sensitively. Moreover, the selective and competitive fluorescent experiments show that DW1 is highly selective for recognition of Zn2+ over other common metal ions. Spectrophotometric titration and ESI-MS spectrum indicate the formation of a 1∶1 DW1-Zn2+ complex and the association constant K was calculated as 1.75×104 (mol/L)-1.