同步荧光法具有选择性好、 灵敏度高、 干扰少等特点, 可用于多组分多环芳烃混合物的同时测定, 本文建立了恒定波长同步荧光光谱法同时测定水中萘和菲的新方法。 研究了萘和菲在不同溶剂中的荧光光谱特性, 确定了同步荧光的最优波长差。 当Δλ=100 nm, 萘和菲激发波长(λex)分别为220.2和248.8 nm时, 在0.5～25.0 μg·L-1浓度范围内, 荧光强度与浓度呈现良好的线性关系, 相关系数分别为0.999 5和0.999 7; 萘和菲检出限均低于0.03 μg·L-1, 回收率在98.0%～101.5%。 该方法方便快捷, 预处理简单, 可用于水中萘、 菲的快速测定。

In view of synchronous fluorescence possessing the character of good selectivity, high sensitivity, less interference, etc. it can be used for simultaneous determination of multi-component mixtures of polycyclic aromatic hydrocarbons(PAHs). A new method of constant-wavelength synchronous fluorescence spectrometry to determine two naphthalene and phenanthrene of PAHs was developed in this study. The effect of different experimental conditions, such as different disolvents for character of fluorescence spectra and the choose of the optimal wavelength difference were studied. Experiment showed that the simultaneous indentification and quantitative determination of the two PAHs when Δλ=100 was chosen. The fluorescence intensity was linearly related to naphthalene and phenanthrene concentration in the range of 0.5～25.0 μg·L-1 with correlation coefficient 0.999 5 and 0.999 7, respectively. The detection limits were all lower than 0.03 μg·L-1, and the relative standard deviations for naphthalene and phenanthrene were 1.19% and 180%(n=7), respectively. Results show that the compounds can be analyzed qualitatively and quantitatively by synchronous fluorescence spectrometry.