采用荧光光谱结合组织病理学方法, 研究脉冲电磁场辐照后大鼠脑组织结构的变化情况, 探讨血清荧光光谱变化与脑损伤的关系。 将雄性SD大鼠随机分为假辐照组和辐照组, 辐照组暴露于宽频脉冲电磁场, 在辐照后5个不同时间点采集血样, 分析血清荧光光谱和S100B蛋白表达情况, 并观察脑组织病理切片。 结果发现, 脑损伤程度和辐射剂量有一定的关系, 辐射剂量越大, 损伤越严重, 其变化趋势和S100B蛋白表达、 脑组织病理观察结果一致。 说明血清荧光光谱法可能适用于对电磁脉冲作用后生物体和脑损伤效应的分析。

To investigate the relationship between the fluorescence spectra of serum and the brain injury effect, the alteration of fluorescence emission in serum was collected by fluorescence spectroscopy, the pathologic changes in the rat brain were obvious by histopathology after exposure to PEMF. Sprague-Dawley male rats were randomized into sham exposed and PEMFs exposed groups. After exposure to PEMF(3.5 ns rising time, 14 ns pulse width, and amplitude up to 200 kV/400 kV at 1 Hz repetitive rate) at 0.5, 1, 3, 6 and 12 h, the expression of S100B and the fluorescence spectra in serum were detected, the changes in brain morphology were observed. The results showed that the fluorescence intensity of 400 kV groups were higher than 200 kV groups at different time points after exposure to PEMF. It suggests that the extent of brain injury was associated with the pulse frequency. The trends of fluorescence spectra in serum coincide with the expression of S100B and pathologic changes. It shows that fluorescence spectroscopy could apply to analysis of the effect of brain injury after exposure to PEMF.