利用傅里叶变换红外光谱(FTIR)研究了水稻、 玉米、 蚕豆正常叶和稻瘟病叶、 玉米和蚕豆锈病叶。 结果显示, 其红外光谱均主要由多糖、 蛋白质的振动吸收谱带组成。 光谱整体相似, 差异主要表现为光谱峰位、 峰形及吸收强度比。 三种作物正常叶的二阶导数光谱在1 750～1 500 cm-1范围内的相关系数差异明显。 水稻正常叶与稻瘟病叶的吸收强度比A1 056/A1 652; 蚕豆正常叶与锈病叶的吸收强度比A1 652/A2 920; 玉米正常叶和锈病叶(非病斑部位)吸收强度比A1 056/A2 920, 玉米正常叶和玉米锈病叶(病斑处)吸收强度比A1 652/A2 920, 均呈现出病变叶吸收强度比正常叶要小的趋势。 表明他们之间的多糖和蛋白质的含量存在差异。

Fourier transform infrared Spectroscopy (FTIR) was used to study healthy and diseased leaves of rice, corn, and broad bean. The results show that the infrared spectra of the leaves are mainly composed of the absorption bands of proteins and polysaccharide. The spectra are similar, but the differences were observed in the peak positions, shapes, and absorption ratios. The remarkable differences in correlation coefficients were also observed in the region of 1 800~1 000 cm-1 of the second derivate spectra. The absorption ratio A1 056/A1 652 of healthy rice leaves and rice blast leaves, A1 652/A2 920 of healthy broad bean leaves and leaves, A1 056/A2 920 of healthy corn and corn rust (non-lesion site), and A1 652/A2 920 of healthy corn and corn rust (lesion site) all show a decline from healthy to diseased leaves, showing that the contents of polysaccharide and the protein have some differences.