中国激光, 2012, 39 (11): 1106003, 网络出版: 2012-09-26
四路频分复用荧光共焦显微探测系统实验研究
Experimental Study of Four-Channel Frequency Division Multiplexed Fluorescence Confocal Microscopy
显微 共焦显微 频分复用 荧光信号 细胞探测 布拉格光栅 microscopy confocal microscopy frequency division multiplexing fluorescence signal cell detection Bragg grating
摘要
报道了采用全息聚合物分散液晶(H-PDLC)布拉格光栅阵列斩波调制的四通道频分复用荧光共焦显微探测系统的实验研究。通过高衍射率H-PDLC布拉格光栅将单束激光分成四束激发光并进行不同频率的载频调制,将激发光聚焦到生物样品上产生荧光信号并采集后,再通过傅里叶变换、滤波和解调,最后将采集到的信号还原成四路荧光信号强度随时间变化的曲线。实验搭建了激发光中心波长为405 nm的四路频分复用荧光探测系统,并成功探测到鼠神经海马细胞样品中激发的四点荧光信号的图像以及强度变化信息。
Abstract
We report an experimental study of four-channel frequency division multiplexed fluorescence confocal microscopy (FDMFCM) detection system modulated by holographic polymer dispersed liquid crystal (H-PDLC) Bragg grating array chopper. In the system, fluorescence signal is excited by the exciting light focused on the biological sample, and then is detected. Fourier transform, filtration and demodulation are then carried out to restore the fluorescence signal with a curve of four-channel fluorescence signal intensity versus time. The four-channel FDMFCM system is experimentally set up with 405 nm laser as the excitation light, and the image and intensity information of the fluorescence signal, which are excited from the mouse neural hippocampus cell sample, are successfully detected.
唐平玉, 郑继红, 孙国强, 张梦华, 郭彩虹, 庄松林. 四路频分复用荧光共焦显微探测系统实验研究[J]. 中国激光, 2012, 39(11): 1106003. Tang Pingyu, Zheng Jihong, Sun Guoqiang, Zhang Menghua, Guo Caihong, Zhuang Songlin. Experimental Study of Four-Channel Frequency Division Multiplexed Fluorescence Confocal Microscopy[J]. Chinese Journal of Lasers, 2012, 39(11): 1106003.