光学仪器, 2011, 33 (3): 57, 网络出版: 2012-12-06   

基于数字微镜的共焦显微系统的光路设计

The optical design of confocal microscope based on DMD
作者单位
上海理工大学 光电信息与计算机工程学院,上海200093
摘要
详细叙述了共焦技术中的横向扫描技术,介绍了基于数字微镜(DMD)的共焦显微镜结构与原理,建立了基于DMD的并行检测系统,并且进行了光路的优化设计。实验结果表明,采用传统共焦显微镜光路时,光线出射分光棱镜时存在棱镜内表面反射问题,导致DMD上同一像素块在CCD上成两个像。在此分析了上述现象产生的原理,给出了解决此问题的方法。
Abstract
It described horizontal scanning technologies of confocal technology in detail and introduced the structure and principle of confocal microscope based on digital micromirror device (DMD). Besides, the parallel detection system based on DMD was established, and the optical path design was optimized as well. It was found that in the normal optical system there was reflection on the inner surface of prism, which causes double images on CCD. Based on theoretical analysis and repeated experiments, spectral plates were taken the place of splitting prism and the refection on inner surface of prism was eliminated. A promoted optical system is achieved.

官志超, 张运波, 侯文玫. 基于数字微镜的共焦显微系统的光路设计[J]. 光学仪器, 2011, 33(3): 57. GUAN Zhichao, ZHANG Yunbo, HOU Wenmei. The optical design of confocal microscope based on DMD[J]. Optical Instruments, 2011, 33(3): 57.

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