应用傅里叶变换红外光谱技术对七种常绿树的幼叶、成叶和老黄叶进行了研究, 以期探讨常绿树叶自然衰老的光谱规律。 不同时期叶片红外光谱变化主要在1 800～700 cm-1范围, 用吸光度比A1 070/A2 927和A1 070/A1 160考察叶样多糖相对含量变化, 用A1 318/A2 922考察草酸钙相对含量变化, 用1 800～1 500 cm-1范围拟合峰的峰高比H1 650/H1 740考察蛋白质相对含量变化。 结果显示在自然衰老过程中蛋白质、 糖类、 草酸钙相对含量有变化, 但不同种树变化规律不同, 说明不同种树叶衰老过程中物质积累、 动员规律不同。 证明中红外光谱可用于植物衰老生理变化的研究。

In order to investigate plant physiological process of leaf senescence and aging, Fourier transform infrared (FTIR) spectroscopy was used to study the young, mature, and old yellow leaves from seven species of evergreen trees. The spectra of the leaves from different growing period are different in the region of 1 800～700 cm-1. The absorption ratios A1 070/A2 927, A1 070/A1 160 were used to evaluate the relative changes of polysaccharides, and A1 318/A2 922 was used to estimate the change of calcium oxalate during leaf senescence. Decomposition and curve-fitting analysis was performed in the region of 1 800～1 500 cm-1. The sub-band absorption ratio H1 650/H1 740 was used to evaluate the relative changes of protein in the leaves. The results show that the accumulation and mobilization of polysaccharides, protein, and calcium oxalate during leaf growing period were different in different plant species. This study demonstrates the potential of mid-infrared spectroscopy for investigation of plants senescence, as well as physiological and biochemical changes of plants.