荧光内滤效应以及样品池中荧光物质对激发光的吸收分布会直接影响荧光谱的强度和谱形, 共同制约荧光分析法的应用。 使用吸收谱及荧光谱存在交迭的双组分混合溶液, 研究一种新的基于物理吸收模型的校正方法, 以校正荧光内滤效应及吸收分布对荧光强度的影响。 对三聚噻吩和五聚噻吩混合液的光谱研究表明, 使用所述校正方法可以达到较为理想的校正效果, 误差小于5%。

The inner filter effect (IFE) and the absorption depth from the fluorescence material will directly lead to an artifact for intensity and spectral shape of the fluorescence spectra, and they restrict the application of fluorescence analysis. A two-component mixed solution with overlapping absorption spectra was used to develop a new method based on physical absorption model to correct the influences of IFE and the absorption depth on fluorescence intensity. The spectral investigations of terthiophene/quinquethiophene mixture solution show that a good correction result can be obtained by using the above correction method, and the error after correction is less than 5%.