光学学报, 2013, 33 (5): 0524001, 网络出版: 2013-04-09   

表面等离子体共振成像系统相位提取

Extracting Phase Information of Surface Plasmon Resonance Imaging System
作者单位
1 南开大学信息技术科学学院现代光学所, 天津 300071
2 南开大学人民医院, 天津 300121
摘要
表面等离子体共振(SPR)生物传感器应用于生物大分子结合反应的实时测量时,为满足高通量检测的要求,SPR 传感器应具有宽场成像检测的能力。建立了适用于以马赫曾德尔结构为基础的差分干涉SPR成像系统,该结构能够有效降低环境噪声,并对其提取相差的算法进行研究。在信号光与参考光合束之后,通过沃拉斯顿棱镜将p光与s光分开,经透镜聚焦后进入CCD,通过互相关运算使两光斑对准,相位从p光与s光干涉光强的若干变化周期中提取,同时通过取平均减小噪声影响,进行点对点相减得到相变值,并以此方法进行扫描得到相变图。系统通过时间轴提取相位信息,不损失空间分辨率,每10秒探测1次数据,空间分辨率为31.25 μm×20.83 μm,折射率分辨率为10-5RIU。
Abstract
There is a request of high throughput measurement when surface plasmon resonance (SPR) biosensors are applied to real-time measure binding reaction of bio-molecular, so SPR biosensors need wide-field imaging ability. A differential interferometry SPR imaging biosensor based on Mach-Zehnder configuration which can decrease environment noise effectively is set up. An algorithm is created to extract phase information in differential phase-sensitive SPR imaging biosensor. After signal and reference beams are combined, p and s polarization beams are splitted by a Wollaston prism and received by CCD after focused by lens. A correlation algorithm is used to align these two light spots. The phase is extracted from several periods of p and s polarization interference intensity changes and the noises decreases simultaneously by the average. The differential phase is calculated by point to point subtraction. The differential phase image is obtained by scanning in this way. For phase information is extracted from time axis in the system, there isn't decrease exist in spatial resolution. The system detects one data per 10 s. Spatial resolution of the system is 31.25 μm×20.83 μm and refractive index resolution is 10-5RIU.

王弋嘉, 张崇磊, 王蓉, 朱思伟, 袁小聪. 表面等离子体共振成像系统相位提取[J]. 光学学报, 2013, 33(5): 0524001. Wang Yijia, Zhang Chonglei, Wang Rong, Zhu Siwei, Yuan Xiaocong. Extracting Phase Information of Surface Plasmon Resonance Imaging System[J]. Acta Optica Sinica, 2013, 33(5): 0524001.

本文已被 6 篇论文引用
被引统计数据来源于中国光学期刊网
引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!