Chinese Optics Letters, 2013, 11 (s1): S10402, Published Online: May. 30, 2013
Design and preparation of filter sets for fourplex fluorescence PCR instruments Download: 563次
Abstract
Aiming at the crosstalk problem caused by small spectral intervals between fluorescent reagents in fourplex fluorescence quantitative polymerase chain reaction (PCR) detection analysis system, we calculate and analyse the effect of cut-off steepness, central wavelength positioning, and bandwidth of filters on crosstalk. Design and prepare four sets of fluorescence excitation and emission filters with proper shape cut-off steepness (optical density (OD) from OD 0.3 to OD 6 is less than 8 nm) and bandwidth (9–11 nm). In fourplex PCR instrument, crosstalk coefficient in all four channels are less than 0.3%.
Shuaifeng Zhao, Xiaojun Yin, Yi Cao, Peng Lei, Ruisheng Wang, Shuguo Fei. Design and preparation of filter sets for fourplex fluorescence PCR instruments[J]. Chinese Optics Letters, 2013, 11(s1): S10402.