根据离体细胞光动力疗法实验研究对光源的基本要求，设计了一种基于LED的新型光源。以卟啉类光敏剂为应用对象，采用波长为627 nm的LED作为光源，利用TracePro对多个LED组成阵列光源后的光分布进行数值模拟，优化LED阵列的排列方式和间距，并对LED阵列光源进行二次光学设计。在数值模拟的基础上，设计并成功开发了应用于离体细胞光动力疗法实验研究的LED光源样机。测量结果表明：LED光源辐照在标准细胞培养板区域内的平均功率密度为13.7 mW/cm2，照度均匀性达到90%，可以满足离体细胞光动力疗法实验研究对光源的基本要求，有望得到推广应用。

A novel LED-based light source is developed for the photodynamic therapy (PDT) treatment of cells in vitro. According to the characteristics of porphyrin-based photosensitizers for PDT applications, the red LED with emission wavelength of 627 nm is chosen. The arrangement mode and the distance between two LED units are optimized based on the simulation of light distribution for LED array by using TracePro software. Furthermore, the secondary optical design is performed in order to further improve the illumination uniformity. Based on the mathematical simulation, a novel LED-based light source is successfully developed, and the average power density and illumination uniformity are measured to be around 13.7 mW/cm2 and 90%, respectively. The obtained results imply that the LED-based light source is a convenient and promising light source for in vitro PDT studies.