光谱学与光谱分析, 2013, 33 (6): 1496, 网络出版: 2013-06-07
结直肠癌细胞株及细胞核的傅里叶变换红外光谱研究
Investigations on Colorectum Cancer Cell Lines and Nucleus by FTIR Spectroscopy
摘要
运用傅里叶变换红外光谱技术研究了正常组织(30例)和细胞株(SW 620), 正常细胞核和癌变细胞核的红外光谱特征, 为红外光谱诊断结直肠癌奠定了细胞及亚细胞水平的实验基础。 将所得光谱图的峰位及峰强比进行统计分析发现: 细胞株(SW 620)和癌变细胞核中谱带(2 925, 1 240和1 085 cm-1)的峰位均向高波数移动(p<0.05), 而谱带1 400 cm-1的峰位均向低波数移动(p<0.05); 细胞株(SW 620)中峰强比(I1 650/I1 460, I1 400/I1 460, I1 240/I1 460,)较正常组织升高(p<0.05), 而I1 740/I1 460则降低(p<0.01)。 癌变细胞核中(I1 650/I1 460, I1 400/I1 460, I1 240/I1 460)较正常细胞核亦升高(p<0.05).这些差异可作为区别组织良恶性的指标。
Abstract
The purpose of this study is to establish the cell and subcellular basis for the diagnosis of colorectal cancer by researching the FTIR( Fourier transform infrared spectra) of the normal tissue (30 cases) and cell line (SW 620), normal nuclei and malignant nucleus with Fourier transform infrared spectrum technology. The results of the peak position and the relative intensity obtained by statistical analysis indicate that the bands at 2 925, 1 240 and 1 085 cm-1 shifted toward higher wavenumber (p<0.05) in the FTIR spectra of cell lines (SW 620) and malignant nucleus. The 1 400 cm-1 band shifted toward lower wave number (p<0.05). The relative intensity ratios of I1 650/I1 460, I1 400/I1 460 and I1 240/I1 460 increased in the FTIR spectra of cell lines (SW 620) compared with normal tissue (p<0.05), but inversely, I1 740/I1 460 decreased significantly (p<0.01). The relative intensity ratios of I1 650/I1 460, I1 400/I1 460 and I1 240/I1 460 also increased in the FTIR spectra of the malignant nucleus. The above statistic differences are the basis for diagnosing colorectum cancer by FTIR spectroscopy.
张超, 孙学军, 刘栋, 刘微, 郑见宝, 杜俊凯, 凌晓峰, 张元福, 徐怡庄, 吴瑾光. 结直肠癌细胞株及细胞核的傅里叶变换红外光谱研究[J]. 光谱学与光谱分析, 2013, 33(6): 1496. ZHANG Chao, SUN Xue-jun, LIU Dong, LIU Wei, ZHENG Jian-bao, DU Jun-kai, LING Xiao-feng, ZHANG Yuan-fu, XU Yi-zhuang, WU Jin-guang. Investigations on Colorectum Cancer Cell Lines and Nucleus by FTIR Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2013, 33(6): 1496.