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基于本征荧光测量的双通道生物气溶胶检测技术研究

Research on Dual-Channel Detection Technology of Bio-Aerosols with Intrinsic Fluorescence Measurement

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摘要

近年来,大气生物气溶胶的实时监测技术已经成为研究领域的热点。在已有技术的基础上,研发出了含有280 nm和365 nm两种激发光波长的双通道生物气溶胶检测系统。利用该检测系统,分别测量了色氨酸、烟酰胺腺嘌呤二核苷酸(NADH)的荧光光谱和强度,确定了对该系统两个通道进行标定的方法,并结合对牛血清蛋白(BSA)以及易引起误判的香烟烟雾等物质的测量结果进行了分析与探讨。实验结果证明了通过多通道紫外光诱导生物气溶胶粒子产生本征荧光,并对荧光光谱与强度等信息进行分析可以较为准确地实现对粒子种类的预判别。这种技术具有较强的应用价值与发展空间。

Abstract

Real-time detection of bio-aerosol has become the focus of research in recent years. Based on the existing technology, the dual-channel bio-aerosol detection method which includes 280 nm and 365 nm channel is developed. With the dual-channel bio-aerosol detection system, the intrinsic fluorescence spectra and intensity of tryptophan and nicotinamide adenine dinucleotide (NADH) are detected respectively by deploying the ultraviolet source at 280 nm and 365 nm. On the basis of the result, the calibration method is determined, and then the analysis of bull serum albumin (BSA) and cigarette particles′ results are researched. The results demonstrate that it can improve the accuracy of particles pre-discrimination by using the dual-channel bio-aerosol detection technology. The technology has strong application value and development space.

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中图分类号:TN247

DOI:10.3788/aos201333.0812005

所属栏目:仪器,测量与计量

基金项目:上海市自然科学基(11ZR1441700)

收稿日期:2013-01-14

修改稿日期:2013-04-10

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作者单位    点击查看

徐傲:中国科学院上海光学精密机械研究所, 上海 201800中国科学院大学, 北京 100049
熊超:中国科学院上海光学精密机械研究所, 上海 201800中国科学院大学, 北京 100049
张佩:中国科学院上海光学精密机械研究所, 上海 201800中国科学院大学, 北京 100049
廖小情:中国科学院上海光学精密机械研究所, 上海 201800
杨巍:中国科学院上海光学精密机械研究所, 上海 201800
赵永凯:中国科学院上海光学精密机械研究所, 上海 201800
黄惠杰:中国科学院上海光学精密机械研究所, 上海 201800

联系人作者:徐傲(xuao20060793@163.com)

备注:徐傲(1989—),男,硕士研究生,主要从事生物气溶胶光电检测技术方面的研究。

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引用该论文

Xu Ao,Xiong Chao,Zhang Pei,Liao Xiaoqing,Yang Wei,Zhao Yongkai,Huang Huijie. Research on Dual-Channel Detection Technology of Bio-Aerosols with Intrinsic Fluorescence Measurement[J]. Acta Optica Sinica, 2013, 33(8): 0812005

徐傲,熊超,张佩,廖小情,杨巍,赵永凯,黄惠杰. 基于本征荧光测量的双通道生物气溶胶检测技术研究[J]. 光学学报, 2013, 33(8): 0812005

被引情况

【1】高颜,李军生,李蔚仑,黄国霞,闫柳娟. 茜草色素蒽棕与DNA的相互作用机理研究. 光学学报, 2015, 35(2): 230003--1

【2】饶志敏,华灯鑫,何廷尧,乐静. 激光诱导生物气溶胶荧光雷达的设计与数值仿真. 光学学报, 2015, 35(10): 1028001--1

【3】李红霞,钮洁青,陈敬蓉,宋寒松,曹静. 荧光成像无损显现纸张潜指印研究. 激光与光电子学进展, 2016, 53(2): 23002--1

【4】王强,何廷尧,华灯鑫. 不同紫外波段激发生物气溶胶荧光雷达系统性能分析. 光子学报, 2017, 46(12): 1201002--1

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