Chinese Optics Letters, 2013, 11 (9): 091701, Published Online: Aug. 27, 2013
Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells Download: 615次
170.0170 Medical optics and biotechnology 140.7010 Laser trapping 180.0180 Microscopy 120.0120 Instrumentation, measurement, and metrology
Abstract
We carry out in situ single-molecule measurements of the specific interaction between apolipoprotein A-I (apoA-I) and ATP binding cassette transporter A1 (ABCA1) on THP-1 cells. Single-molecule force spectroscopy shows that similar to normal apoA-I, the dysfunctional apoA-I from diabetes patients interacts with ABCA1 via two different binding sites on the cells. The strength of dysfunctional apoA-I binding to a high-capacity binding site is 26.5+(-)4.9 pN. The minor direct apoA-I/ABCA1 binding strength is 56.7+(-)4.1 pN. These results facilitate a pathological understanding of the mechanisms that underlie the specific interaction of apoA-I and ABCA1 at the single-molecule level.
Jie Yu, Xunliang Tong, Chengbin Li, Yining Huang, Anpei Ye. Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells[J]. Chinese Optics Letters, 2013, 11(9): 091701.