光谱学与光谱分析, 2013, 33 (9): 2512, 网络出版: 2013-09-30
秦岭大熊猫主食竹叶片不同花期内源激素变化及与高光谱的响应关系
Responding Relationship between Endogenous Hormones and Hyperspectra Reflectance for the Different Flowering Stages of the Giant Panda’s Staple-Food Bamboos in Qinling
大熊猫主食竹开花 内源激素 原始高光谱反射 光谱反射一阶导数 响应关系 Flowering of the Giant Panda’s staple-food bamboo Endogenous hormones Original hyper-spectra reflectance First derivative of spectral reflectance Responding relationship
摘要
选取佛坪保护区不同开花状态的大熊猫主食竹种巴山木竹(Bashania fargesii)、 秦岭箭竹(Fargesia qinlingensis)和龙头竹(Fargesia dracocephala)进行高光谱测定, 并利用酶联免疫吸附法测定分析竹叶的内源激素(赤霉素、 生长素、 玉米素核苷和脱落酸)的差异, 并建立关系模型。 结果为: (1)巴山木竹和龙头竹在未开花状态下的赤霉素含量高于开花状态下的, 而秦岭箭竹相反; 三种竹子未开花竹的生长素和脱落酸含量都低于开花竹; 未开花竹的玉米素核苷高于开花竹的。 (2)除巴山木竹的生长素和玉米素核苷含量与一定波段的原始光谱显著相关, 三种竹子的其他激素含量仅与光谱一阶导数在一定波段极显著相关。 (3)利用特征波段得到的线性模型精度高。 研究表明激素含量与光谱的相关性能预测竹子开花。
Abstract
The present research aims at utilizing hyperspectra information to estimate the content of endogenous hormones for predicting the bamboo flowering. The authors selected the giant panda’s staple-food bamboos Bashania fargesii, Fargesia qinlingensis and Fargesia dracocephala in different flowering situations to measure their hyperspectral reflectance in Foping Nature Reserve. The enzyme-linked immunosorbent assay was used to measure the contents of four endogenous hormones: Gibberellin (GA3), Auxin (IAA), Zeatin nucleotide (Zr) and Abscisic acid (ABA), and non-parametric test was applied to analyze their differences. Then, we built the responding relationship models between hormones and hyperspectral reflectance. The results showed that (1) GA3 contents of B. fargesii and F. dracocephala were higher in the flowering bamboo than in the non-flowering bamboo, while F. qinlingensis showed the opposite result; IAA and ABA contents of three bamboos were all lower in non-flowering bamboo than in the flowering one; Zr contents for three bamboo species were higher in the non-flowering bamboo than in the flowering one. (2) Except that B. fargesii’s IAA and Zr had significant relationships with original spectral reflectance among some bands, other hormones of bamboos all showed the poor relationships with the original spectra; however, some specific bands of all four hormones of three bamboos had the very significant relationships with the first derivative reflectance. (3) The precisions of linear models were higher than the Parabola models. The authors concluded that the close relationships between the hormones’ contents and spectra reflectance can be used to forecast the flowering of panda bamboos.
刘雪华, 吴燕. 秦岭大熊猫主食竹叶片不同花期内源激素变化及与高光谱的响应关系[J]. 光谱学与光谱分析, 2013, 33(9): 2512. LIU Xue-hua, WU Yan. Responding Relationship between Endogenous Hormones and Hyperspectra Reflectance for the Different Flowering Stages of the Giant Panda’s Staple-Food Bamboos in Qinling[J]. Spectroscopy and Spectral Analysis, 2013, 33(9): 2512.