基于表面增强拉曼光谱的成像分析方法具有频带窄, 水溶液背景弱, 稳定性好, 高特异性等优势已成为生物成像领域的优良选择。拉曼成像技术拓展了拉曼光谱的应用范围, 使其不再只是检测单点化学成分的手段, 而进一步用于对评价区域内化学物质成分、分布及变化进行整体统计和描述。本文探讨了表面增强拉曼散射的原理及增强机制, 介绍了基于表面增强拉曼光谱的拉曼成像技术, 并对其在无标记成像及带标记成像中的细胞成像、活体成像, 特别是其在生物医学方面的应用进行了详细论述, 最后讨论了表面增强拉曼光谱生物成像技术存在的问题, 展望了该项技术的研究和应用前景。

The bioimaging technology based on the Surface-enhanced Raman Spectroscopy(SERS) has emerged as a potential alternative due to its narrow bandwidth, weak background interference, high stability of label agent and specificity. Raman imaging technique can not only analyze a single point of the sample, but also offer an integral description of ingredient, distribution and variation for a large scale selected region. This paper introduces the principle of surface enhanced Raman scattering and its enhancement mechanism. Then, it reviews the recent development of SERS based bioimaging where both non-labeled and labelled SERS imaging techniques are included, and describes its applications to the biomedicine. Finally, it discusses some problems existed in the current technology and points out the future developing trends of the SERS-based bioimaging.