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金纳米棒核/二氧化硅壳纳米复合结构的可控制备及细胞成像

Controlled synthesis and cell imaging of gold nanorod-silica core-shell nanoparticles

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摘要

采用模板合成以及溶胶凝胶方法制备了金纳米棒核/二氧化硅壳(GNR@SiO2)纳米复合粒子, 探讨了这种新型纳米复合结构的可控制备、光谱性质、细胞毒性和细胞成像。通过紫外可见分光光度计、透射电镜、共聚焦显微镜对样品进行表征, 结果表明: 通过对反应时间的调控, 获得的纳米复合粒子的二氧化硅壳层厚度可以控制在20~30 nm。由于二氧化硅壳层的存在, 大大提高了金纳米棒的稳定性, 同时降低了金纳米棒的细胞毒性; 此外, 由于二氧化硅壳层具有良好的化学修饰作用, 因此可以将荧光探针分子标记在二氧化硅壳层表面, 修饰后的纳米复合粒子可以通过细胞内吞作用进入细胞, 从而实现细胞内的光学成像。因此, 该纳米粒子复合材料在生物传感、细胞成像以及光热治疗等方面有着良好的应用前景。

Abstract

The template method and sol-gel technique were used for the preparation of gold nanorod-silica core-shell (GNR@SiO2) nanocomposites. The controllable synthesis, spectroscopic properties, cytotoxicity and cell imaging of these nanocomposites were discussed by using UV-Vis spectrophotometry, transmission electron microscopy, confocal microscopy and other characterized measurements. The results show that, by control of the reaction time, the thickness of silica shell can be facilely tuned in the regime of 20-30 nm; the colloidal stability of gold nanorods has been greatly improved, and their cell cytotoxicities are significantly decreased due to the presence of the silica shell. In addition, fluorescent probes can be easily attached on the silica shell by post chemical reactions. As formed fluorescent core-shell nanocomposites can be easily captured by cells through endocytosis pathway, which is useful for the optical imaging. Therefore, these nanoparticle composites have a great potential in fields of biosensors, cell imaging and photothermal therapy.

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中图分类号:O482.3;TB383

DOI:10.3788/co.20130605.0743

所属栏目:光学功能材料

基金项目:国家自然科学基金资助项目(No.91123029,No.61077066, No.81201804); 吉林省自然科学基金资助项目(No.20101534); 吉林省科技发展计划资助项目(No.201205006)

收稿日期:2013-07-12

修改稿日期:2013-09-17

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王英帅:吉林大学 集成光电子国家重点联合实验室, 吉林 长春 130012
周颖:吉林大学 集成光电子国家重点联合实验室, 吉林 长春 130012
王珺楠:吉林大学 白求恩第二医院 心血管内科, 吉林 长春 130041
邵丹:吉林大学 白求恩医学院 药理系, 吉林 长春130021
孙允陆:吉林大学 集成光电子国家重点联合实验室, 吉林 长春 130012
李晶:吉林大学 白求恩医学院 药理系, 吉林 长春130021
董文飞:吉林大学 集成光电子国家重点联合实验室, 吉林 长春 130012

联系人作者:王英帅(yingshuaiwang1987@163.com)

备注:王英帅(1986—), 男, 山东潍坊人, 硕士, 2009年于吉林大学获得学士学位, 主要从事金纳米粒子在诊治一体化方面的研究。

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引用该论文

WANG Ying-shuai,ZHOU Ying,WANG Jun-nan,SHAO Dan,SUN Yun-lu,LI Jing,DONG Wen-fei. Controlled synthesis and cell imaging of gold nanorod-silica core-shell nanoparticles[J]. Chinese Optics, 2013, 6(5): 743-749

王英帅,周颖,王珺楠,邵丹,孙允陆,李晶,董文飞. 金纳米棒核/二氧化硅壳纳米复合结构的可控制备及细胞成像[J]. 中国光学, 2013, 6(5): 743-749

被引情况

【1】李欣远,纪穆为,王虹智,涂国鹏,万晓冬,刘佳佳,刘 佳,徐 萌,张加涛. 近红外光热转换纳米晶研究进展. 中国光学, 2017, 10(5): 541-554

【2】修景锐,胡思怡,李金华,任 升,刘丽炜. 基于近红外量子点的荧光共振能量转移生物探针构建及应用. 中国光学, 2018, 11(1): 74-82

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