光学学报, 2014, 34 (1): 0129002, 网络出版: 2014-01-02
动态光散射图像法测量纳米颗粒粒度研究
Nanoparticle Sizing by Image Processing with Dynamic Light Scattering
摘要
提出了一种基于动态光散射原理的图像法测量纳米颗粒粒径的新方法,采用面阵CCD数以万计的像素同时并行测量处于布朗运动的纳米颗粒空间分布的动态散射光信号,对测得的信号进行数据处理,得到了纳米颗粒粒径。对27、79、482、948 nm 4种不同粒径的纳米标准颗粒进行了实验研究,针对面阵CCD拍摄帧率远低于光电倍增管测量频率的特点,采用质量分数为55%高粘度甘油水溶液作为分散介质,在CCD拍摄帧率为8290 frame/s时,27 nm颗粒的测量误差从以水为分散介质时的15.1%降至1.9%。与目前动态光散射纳米颗粒测量方法相比,该方法大幅度减少了测量时间,仅为现有方法的1%以下,并可大幅度简化测量装置。
Abstract
Image processing for nanoparticle sizing with dynamic light scattering (DLS) technique is introduced. A CCD is used to capture the spatial distribution of the scattered light by nanoparticles under Brownian motion. The sizes of the nanoparticles can be determined with the post-processing of the recorded data by calculating the autocorrelation functions of the scattered light. Standard nanoparticles of four different sizes (27, 79, 482, 948 nm) are used in the experiments. Since the frame rate of CCD is far lower than the frequency of photomultiplier, 55% glycerol/water emulsion is employed as disperse medium here, therefore. The measurement deviation is dramatically reduced from 15.1% to 1.9% in 27 nm nanoparticles at frame rate of 8290 frame/s. Compared with conventional DLS, this technique is advantageous due to the compact and simple measurement system with short measurement time which is 1% of conventional DLS measurement time.
王志永, 蔡小舒, 徐呈泽, 刘丽丽. 动态光散射图像法测量纳米颗粒粒度研究[J]. 光学学报, 2014, 34(1): 0129002. Wang Zhiyong, Cai Xiaoshu, Xu Chengze, Liu Lili. Nanoparticle Sizing by Image Processing with Dynamic Light Scattering[J]. Acta Optica Sinica, 2014, 34(1): 0129002.