激光照射对酶活性的影响主要取决于光源的波长和能量。系统研究了激光的波长和能量对酶活性的改变，并且对激光和发光二极管(LED)是否对酶活性改变具有相同的效果也进行了研究。分别采用能量为0~810 J/cm2,激光波长分别为532、808、1064、1342 nm，LED波长分别为640 nm和810 nm的激光照射磷脂酶C，通过高效薄层层析法(HPTLC)测量经过光源照射0.5、1、2、3、4、6、17、24 h后神经酰胺的浓度来研究酶活性的改变。酶活性改变持续时间通过以上实验数据进行评估计算。在一定波长范围内激光和LED照射均可以提高磷脂酶C的活性，效果取决于光源的能量和波长，在经过照射后，酶活性的改变大约可以持续4 h。研究结果表明在报道激光照射对酶活性改变的时候，酶活性改变持续时间应该作为一个主要的激光参数进行声明，还发现照射时选择波长、能量相同的激光源和LED，其对酶活性的改变具有同样的效果。

The influence of laser irradiation on enzyme activity depends on the wavelength and energy of the light. The influence of wavelength and energy on enzyme activity is studied systematically, and whether laser and LED light sources have the same effect on enzyme activity is examined. Phospholipase C is exposed at 0~810 J/cm2 of lasers at the wavelength of 532, 808, 1064, 1342 nm and two LED sources at the wavelength of 640 nm and 810 nm respectively, and enzyme responses are evaluated by measuring ceramide concentration using high performance thin-layer chromatography (HPTLC) after irradiation of 0.5, 1, 2, 3, 4, 6, 17, 24 h. The duration of effect is evaluated from the experimental data. The enzyme activity can be increased by using either laser or LED source whose wavelength is located within a certain range, and the effect depends on the energy and wavelength of the light. The increase in enzyme activity continues for about 4 h after irradiation. The result shows that the duration of enzyme activity change should be included as one of the main laser parameters while reporting the effect of laser irradiation on enzymes. Besides, laser sources and LED sources have the same effect on enzyme activity with the same wavelength and absorbed energy.