利用成像光谱仪图谱合一的特性，研制了一台用于临床诊断及术中导航定位的棱镜-光栅-棱镜式小型医用成像光谱仪。介绍了该成像光谱仪的设计原理，根据应用需求对其光学系统进行了分析，测试了光谱分辨率、谱线弯曲、色畸变等重要光学参数。结果显示，该光谱仪的实际光谱分辨率优于5 nm，满足应用需求。将该仪器与手术显微镜集成，进行了大鼠脑肿瘤在线诊断，获取了大鼠脑部胶质肿瘤的成像光谱数据。通过光谱数据分析，实现了脑肿瘤位置与大小的准确判断。该仪器具有光谱分辨率高、体积小、与手术显微镜有通用接口、可在手术中进行在线诊断等优点。

On the basis of characteristics of imaging spectrometer with imaging-spectral information at the same time, a portable Prism-Grating-Prism(PGP) imaging spectrometer was developed for clinical monitoring and intraoperative navigation. The design principle of the optical system was introduced and analyzed according to the requirements. Then, the principal parameters including spectral resolution, spectral smile, and keystone were tested. The actual spectral resolution was up to 5 nm, which satisfies the needs of clinical application. After combing the imaging spectrometer and an operation microscopy, this paper diagnoses the brain tumors of rats online, and acquires lots of imaging spectral data of brain glioma. By analyzing the spectral data, the position and the size of the tumor is obtained precisely. Obtained results demonstrate that imaging spectrometer developed is characterized by its high resolution and smaller volume and can diagnose tumors in the operation on line for its common interface with a microscopy.