应用激光, 2013, 33 (6): 628, 网络出版: 2014-01-08
人成骨细胞在宽带激光熔覆Nd2O3稀土涂层表面的生物活性
Bioactivity of Human Osteoblasts in the Rare Earths Bioceramic Coating Surface Produced by Wide-Band Laser Cladding
稀土氧化物Nd2O3 宽带激光熔覆 细胞增殖 生物活性 rare earths oxide Nd2O3 wide-band laser cladding cell proliferation bioactivity
摘要
采用宽带激光熔覆技术,通过加入不同含量的稀土氧化物Nd2O3来提高激光熔覆生物陶瓷涂层的生物活性,在TC4钛合金表面制备了含HA+β-TCP的稀土活性梯度陶瓷涂层。使用体外人成骨细胞MG63与Nd2O3稀土活性梯度陶瓷材料共培养,用四甲基偶氮唑盐微量酶反应比色(MTT)法分析细胞增殖情况和通过SEM观察MG63细胞在稀土活性梯度陶瓷涂层材料上的粘附、生长情况。结果表明: Nd2O3稀土活性梯度陶瓷表面的细胞生长旺盛、细胞形态正常,活性涂层细胞相容性良好;涂层表面的HA+β-TCP钙磷基活性陶瓷相数量影响着成骨细胞的增殖,含有Nd2O3的生物陶瓷涂层比TC4钛合金的细胞相容行好,且含有w(Nd2O3)=0.4%、w(Nd2O3)=0.6%的生物陶瓷涂层表面成骨细胞繁殖最快;稀土活性梯度陶瓷材料不仅能够引起细胞的粘附生长,更重要的是要能够促进成骨细胞的定向分化。
Abstract
In this paper, the rare earths active gradient bioceramic coating with HA and β-TCP on TC4 titanium alloy was prepared by using wide-band laser cladding technique. The method was to culture osteoblast on gradient bioceramic coating in vitro. Results show that cells,of Nd2O3 rare earth active gradient ceramic surface cells were normal,active coating had good cytocompatibility; Calcium and phosphorus HA+ β -TCP based bioactive ceramic coating surface phase quantity influences the proliferation of osteoblast, bio ceramic coating containing Nd2O3 than of TC4 titanium alloy cell compatibility good, and contain 0.4wt.%Nd2O3, 0.6wt.%Nd2O3 bioceramic coating on the surface of osteoblasts reproductive fastes; Rare earth activated gradient ceramic material not only can cause the celladhesion and growth, more important is to promote the directional differentiation of bone cells.
李宾, 田兴强, 汪震. 人成骨细胞在宽带激光熔覆Nd2O3稀土涂层表面的生物活性[J]. 应用激光, 2013, 33(6): 628. Li Bin, Tian Xingqiang, Wang Zhen. Bioactivity of Human Osteoblasts in the Rare Earths Bioceramic Coating Surface Produced by Wide-Band Laser Cladding[J]. APPLIED LASER, 2013, 33(6): 628.