利用类囊体溶液的激发光谱和偏振荧光光谱， 研究了温度对光系统Ⅱ(photosystemⅡ, PSⅡ)中色素分子取向的影响。 激发光谱表明在15～45 ℃范围内， 随着温度的升高， 叶绿素a对应的吸收峰发生红移， 激发谱强度在35 ℃达到最大， 65和75 ℃时大大减弱。 类囊体溶液的偏振荧光光谱中， 荧光峰位在15～45 ℃范围内始终保持不变， 计算得到的荧光偏振度随着温度的升高而增加。 分析表明温度通过影响光系统Ⅱ内色素之间以及色素蛋白之间的相互作用， 改变色素排列方向， 调节光合作用效率。 结果可为研究光合过程能量吸收与传递、 光合保护以及太阳能电池材料等方面提供参考。

The effect of temperature on the orientation of pigment in PSⅡwas studied by the fluorescence excitation spectra and polarization fluorescence spectra of spinach thylakoid solution. The experimental results showed that in the temperature range of 15～45 ℃ the absorption band of chl a at 436 nm at room temperature red-shifted with increasing temperature. The excitation spectra intensity reached the maximum at the temperature of 35 ℃, but greatly reduced with temperature at 65 and 78 ℃. In the polarization fluorescence spectra the fluorescence peak of PSⅡ didn’t change with temperature from 15 to 45 ℃. It was also found that the calculated fluorescence polarization degree increased with the temperature in the entire temperature range. The analysis indicated that temperature would affect the orientation of the pigments in PSII and the coupling strength between pigments so to change photosynthetic efficiency. The results will give a certain reference for the study of the energy absorption and transmission, regulation mechanism and also on solar cell materials.