应用荧光光谱仪及微拉曼光谱仪分别对几种常见人工合成 色素溶液的荧光光谱和拉曼光谱进行了实验研究。 一方面,首次测量其不同浓度合成色素溶液的荧光光谱,讨论其谱线特性。另一方面,以有致癌性的合成色素苋菜 红为例,应用拉曼光谱技术进行定性和定量检测,验证拉曼光谱对色素检测的可行性。为了提高探测灵敏度, 以55 nm的金纳米粒子溶胶为基底,在785 nm激发光下对不同浓度的苋菜红溶液进行了表面增强拉曼光谱探测, 探测到的最低浓度为10－17 mol/L。分析结果为这种色素的合理利用及安全快速检测提供了有效的光谱实验依据, 也为其它色素的检测提供了参考方法。

Fluorescence spectrometer and confocalmicro-Raman spectrometer were used to study the fluorescence spectroscopy and Raman spectroscopy of several synthetic food colors. On one hand, the fluorescence spectra of different concentrations were first measured and the spectral characteristics were discussed. On the other hand, the Raman spectroscopy of carcinogenic organic amaranth sample was studied as an example. In order to improve the detection sensitivity, 55 nm gold nanparticles collosol as substrate and 785 nm excitation light were used to study the surface enhanced Raman scattering spectrum of amaranth with different concentrations, and the detected minimum concentration was 10－17 mol/L. The results provide spectrum experiment basis for reasonable utilization of the artificial color and the safe and rapid detection, also provides a new method for safety testing of other artificial color.