光谱学与光谱分析, 2014, 34 (3): 601, 网络出版: 2014-03-14  

光在柑橘组织中的入射深度和分布情况初探

Investigation of Light Penetration Depth and Distribution Inside Citrus Tissue
作者单位
浙江大学生物系统工程与食品科学学院, 浙江 杭州310058
摘要
基于光谱分析的水果内部品质检测技术近年来受到越来越多的关注和应用, 由于水果组织属于混浊介质, 光子在其内部的传播过程非常复杂, 为了从机理上对该方法有更深入的了解, 本文开展了光在柑橘组织中的入射深度和分布情况的实验研究。 通过搭建三维可调实验平台, 采用探针光纤穿刺方式测量光在柑橘组织中的变化情况, 直观观测不同部位组织对光的衰减作用以及光在柑橘内部的变化。 使用632 nm激光和50 W卤钨灯作为光源, 对柑橘内部不同位置处光能量值和透过率两个参数进行探究, 选择差异最为显著的波段分析不同位置处光能量值和透过率的变化趋势, 并运用SPSS软件对不同样品的检测结果进行单因素方差分析, 考察样品因素对检测结果的影响显著性。 研究发现柑橘穿刺光能量值、 透过率与穿刺深度梯度呈正相关, 柑橘果皮及果核相对果肉对光能量有更明显的衰减作用, 且样本因素对检测结果的影响不显著。 本研究方法和结果对进一步开展光在水果内部的传输特性研究有一定的参考价值。
Abstract
Experiment was carried out to explore the light intensity change inside citrus samples in the present study. An experimental platform was set up, including a light box, a spectrometer, a sample stage, an optic fiber probe, light sources, etc. The sample stage is adjustable in three dimensions. The optic fiber probe was used to measure the light changes by observing the light attenuation and intensity variation within the citrus tissues. A 632 nm laser source and a 50 W tungsten halogen lamp light source were used. Light intensity and transmittance were investigated at different positions within the citrus fruit. The band with most significant intensity difference was selected to analyze the light intensity and transmittance trends in different positions inside the citrus fruit. In order to examine the influence significance of the sample factor on test results, SPSS software was used to do the analysis of variation (ANOVA) of different samples. The results showed that light intensity and transmittance have a positive correlation with puncture depth, while citrus peel and stone have a more obvious attenuation effect than citrus flesh, and the influence of the sample factor on the test results is not significant. Further research can be carried out by improving the experimental device. The method used and results obtained in this study are valuable for studies on light transmission properties inside fruit tissue, not only for citrus but also for other kinds of fruits.

吴晨凯, 张亮, 沈黄通, 傅霞萍. 光在柑橘组织中的入射深度和分布情况初探[J]. 光谱学与光谱分析, 2014, 34(3): 601. WU Chen-kai, ZHANG Liang, SHEN Huang-tong, FU Xia-ping. Investigation of Light Penetration Depth and Distribution Inside Citrus Tissue[J]. Spectroscopy and Spectral Analysis, 2014, 34(3): 601.

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