采用红外光谱宏观指纹鉴定的方法对紫锥菊不同部位根、茎、叶、花原药材及醇溶、水溶提取物的一维和二阶导数谱图进行整体分析。原药材谱图显示紫锥菊的各部位都体现淀粉特征峰。醇提物二阶导数谱图给出花中1711 cm-1、1630 cm-1的羧基吸收峰明显强于其他部位, 说明花中多酚类成分高于其他部位。水提物谱图显示, 花、叶中多酚类成分比例相对于茎、根中的含量提高, 而花、叶中硝酸根离子和蔗糖成分比例相对于茎、根中的含量下降。对比整体药材与各部位的谱图, 可以判断出整体药材中所含有的植物部位, 从而达到快速鉴定的目的。

In the present paper, raw radix, stem, leaf and flower of the original medicines and alcohol, soluble extract of introduced Echinacea purpurea Moench were stidied via the IR macro-fringerprint method. The spectra of raw madicinal materials show that all parts show characteristic peaks of starch. In the two-dimensional correlation spectra, the characteristic peak of flower is stronger than that of other parts at 1711 cm-1、1630 cm-1(Carboxylic acid C=O), this proves that the quantity of component of polyphenols in flower is higher than that in the other parts. The FTIR spectra of soluble extract present the component of polyphenols in flower and leaf is higher than in stem and radix, but the component of nitrate and sugar is opposite. Compared the spectra of whole raw medicines with the different parts, it can discern that the whole raw medicines contained which parts of plant, so as to achieve the purpose of rapid identification.