频谱编码成像技术是一种利用光栅在横向位置编码的新型生物组织成像技术。该技术采用一个光栅和一个聚焦透镜在样品上产生光谱编码线，无需额外扫描便可得到一条线的图像，跟频域干涉技术相结合，再加上慢速的线扫描便可获得三维图像，在内窥成像上具有很大的实用价值。在分析频谱编码成像技术基本原理的基础上，推导了主要的性能参数。通过对分辨率测试靶进行成像，验证分析影响横向分辨率的因素。结果表明，编码线方向的分辨率与入射光斑直径成反比，与焦距成正比；垂直于编码线方向分辨率要弱于编码线方向的分辨率。最后对洋葱样品进行成像，验证了频谱编码成像技术的可行性。

Spectrally encoded imaging (SEI) is a new type of tissue imaging technique in which a grating is used to encode the transverse position. In SEI, a diffraction grating and a miniature lens are used to generate a spectrally encoded line on the sample, and a line image can then be obtained without additional mechanical scanning. In combination with Fourier domain optical coherence tomography and slowly line scanning, SEI is able to provide three dimensional image and is a prospective method in endoscopic imaging. Based on analysis of the basic principle of SEI, the typical performance parameters are derived and the factors that affect lateral resolution are tested by imaging of a resolution target. It is demonstrated that the lateral resolution of SEI for the spectrally encoded line is inversely proportional to the incident light diameter and proportional to focal length of the objective lens. It is also found that the resolution in the direction perpendicular to the spectrally encoded line is poorer than that along the sit. Finally, images of onion cells are presented to demonstrate the feasibility of this technique.