基于辣根过氧化物酶( HRP) 催化 H2O2-Luminol系统, 对一种新型酚类衍生物 4-(1,2,4-三氮唑-1-基)苯酚对化学发光的增强作用进行了研究。用HRP标记急性心肌梗死标志物心肌肌钙蛋白Ⅰ(cTnⅠ)单克隆抗体, 通过cTnⅠ的双抗体夹心免疫反应, 建立了简单、灵敏和快速检测人血清中的cTnⅠ含量的增强型酶发光免疫分析方法。实验结果表明, cTnⅠ在1.2 ～ 24 ng/mL浓度范围内与增强型发光强度具有良好的线性关系(R=0.99), 变异系数(n= 8) 为 4.7%。最后, 使用该方法对人血清样品中的cTnⅠ含量进行测定, 测试结果具有较好的稳定性和精度, 能够满足临床检测要求。

Based on the catalyzed oxidation of horseradish peroxidase (HRP) to the hydrogen peroxide and luminol system, a novel p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (TP) was studied to enhance the chemiluminescence. With HRP labeled cardiac troponin Ⅰ (cTn Ⅰ) monoclonal antibody, a simple, sensitive, and rapid enzyme chemiluminescence immunoassay method enhanced by TP for the determination of cTn Ⅰ in human serum samples had been developed by “sandwich type” immune response．The experimental results show that there is a very good linear correlation between the enhanced enzymatic chemiluminescence intensity and the amount of cTn Ⅰ in the range of 1.2 ～ 24 ng/mL (R=0.99), and the coefficient of variation (n=8) is 4.7%. The proposed enhanced enzymatic chemiluminescence immunoassay method had been used successfully for the determination of cTn Ⅰ in human serum.