自动对焦是全自动显微镜的关键技术, 决定着显微镜成像的精确性和实时性。对焦方法包括主动式和被动式两种, 主动式是指基于数字图像处理的对焦方法, 被动式是指基于光学离焦量检测的对焦方法。介绍了两种方法的原理和研究现状, 通过比较其优缺点, 总结出基于离焦误差检测的主动式对焦方法具有更高的精度和速度, 更适用于全自动显微镜系统。

Autofocus is the key technology for automated microscopy. The velocity and accuracy of microscopy is dependent on autofocus methods. There are two types of autofocus technology, the passive type and the active type. The passive type is based on the digital image and the active one is based on the optical defocus error detection . By comparison the principles and development of the two types, it can be concluded that the active type based on the defocus error detection is more fast and accurate, which is more suitable for the automated microscopy.