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功率谱密度法标定光镊的三维光阱刚度

Calibration of Stiffness of Optical Tweezers in Three Dimensions with Power Spectral Density Method

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摘要

采用四象限探测器和功率谱密度法,搭建了一套快速标定光镊三维光阱刚度的测量系统.实验中,用四象限探测器记录微粒做受限布朗运动时的位置信息,用功率谱密度法标定光阱刚度,测得了直径0.97 μm SiO2小球和直径1 μm PMMA小球的光阱刚度与激光功率的关系.结果表明:对于SiO2小球,当激光功率为50~120 mW时,光阱刚度与激光功率成正比;对于PMMA小球,当激光功率为80~130 mW时,光阱刚度与激光功率成正比.该光镊系统可用于生物、物理等微观领域研究的高准确度测力系统.

Abstract

An optical tweezers system which can accurately and quickly calibrate the stiffness of optical tweezers in three dimensions was set up combined with quadrant photodiode detector and power spectral density method. The limited Brownian motion of the trapped particle was recorded by a quadrant photodiode detector, and the stiffness of optical tweezers was calibrated by power spectral density method. The relations of the laser power with the stiffness of a 0.97 μm-diameter silica bead and of the 1 μm-diameter PMMA bead were investigated. The results indicate that the stiffness is proportional to the laser power at the range from 50 mW to 120 mW of laser power for a 0.97 μm-diameter silica bead and at the range from 80 mW to 130 mW for a 1 μm-diameter PMMA bead. This system can be used as a precise force measuring tool for microscopic investigation in biology, physics, etc.

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中图分类号:O439

DOI:10.3788/gzxb20144305.0535001

基金项目:国家重大科学研究计(No. 2012CB921900)和国家自然科学基金(No. 61275193)资)

收稿日期:2013-09-30

修改稿日期:2013-12-10

网络出版日期:--

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梁言生:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室,西安 710119
姚保利:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室,西安 710119
雷铭:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室,西安 710119
于湘华:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室,西安 710119
严绍辉:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室,西安 710119
杨延龙:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室,西安 710119
但旦:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室,西安 710119
郜鹏:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室,西安 710119
闵俊伟:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室,西安 710119
叶彤:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室,西安 710119

联系人作者:梁言生(liangyansheng@opt.cn)

备注:梁言生(1987-),男,博士研究生,主要研究方向为光镊捕获技术.

【1】ASHKIN A, DZIEDZIC M, BJORKHOLM E, et al. Observation of a single-beam gradient force optical trap for dielectric particles[J]. Optics Letters, 1986, 11(5): 288-290.

【2】MANDAL S, SEREY X, ERICKSON D. Nanomanipulation using silicon photonic crystal resonators[J]. Nano Letters, 2010, 10(1): 99-104.

【3】MEHTA A, RIEF M, SPUDICH J, et al. Single-molecule biomechanics with optical methods[J]. Science, 1999, 283(5408): 1689-1695.

【4】DHOLAKIA K, REECE P, GU M. Optical micromanipulation[J]. Chemical Society Reviews, 2007, 37(1): 42-55.

【5】CHU S. Laser manipulation of atoms and particles[J]. Science, 1991, 253(5022): 861-866.

【6】ABBONDANZIERI E, GREENLEAF W, SHAEVITZ J, et al. Direct observation of base-pair stepping by RNA polymerase[J]. Nature, 2005, 438(7067): 460-465.

【7】SEMENOV I, OTTO O, STOBER G, et al. Single colloid electrophoresis[J]. Journal of Colloid and Interface Science, 2009, 337(1): 260-264.

【8】FLORIN L, PRALLE A, STELZER K, et al. Photonic force microscope calibration by thermal noise analysis[J]. Applied Physics A: Materials Science & Processing, 1998, 66: S75-S78.

【9】PENG Fei, YAO Bao-li, LEI Ming, et al. Measurement of optical trapping force and stiffness of micro-particles with the drag-force method[J]. Journal of Opto-electronics·Laser, 2010, 21(1): 78-82.
彭飞, 姚保利, 雷铭, 等. 拖曳法测量微粒光阱力和光阱刚度的实验研究[J]. 光电子·激光, 2010, 21(1): 78-82.

【10】NEUMAN K, BLOCK S. Optical trapping[J]. Review of Scientific Instruments, 2004, 75(9): 2787-2809.

【11】BERG-SΦRENSEN K, FLYVBJERG H. Power spectrum analysis for optical tweezers[J]. Review of Scientific Instruments, 2004, 75(3): 594-612.

【12】UHLENBECK E, OMSTEIN S. On the theory of the Brownian motion[J]. Physical Review, 1930, 36(5): 823-841.

【13】WANG Ming-chen, UHLENBECK E. On the theory of the Brownian motion II[J]. Reviews of Modern Physics, 1945, 17(2-3): 323-342.

【14】GITTES F, SCHMIDT C. Signals and noise in micromechanical measurements[J]. Methods in Cell Biology, 1997, 55: 129-156.

【15】GITTES F, SCHMIDT C. Interference model for back-focal-plane displacement detection in optical tweezers[J]. Optics Letters, 1998, 23(1): 7-9.

【16】PRALLE A, PRUMMER M, FLORIN E, et al. Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light[J]. Microscopy Research and Technique, 1999, 44(5): 378-386.

【17】KUBO R, TODA M, HASHITSUME N. Statistical physics[M]. Heidelberg: Springer, 1985, Vol. 2.

【18】NETO P, NUSSENZVEIG H. Theory of optical tweezers[J]. Europhysics Letters, 2000, 50(5): 702-708.

【19】YAN Shao-hui, YAO Bao-li. Transverse trapping forces of focused Gaussian beam on ellipsoidal particles[J]. Journal of the Optical Society of America B, 2007, 24(7): 1596-1602.

引用该论文

LIANG Yan-sheng,YAO Bao-li,LEI Ming,YU Xiang-hua,YAN Shao-hui,YANG Yan-long,DAN Dan,GAO Peng,MIN Jun-wei,YE Tong. Calibration of Stiffness of Optical Tweezers in Three Dimensions with Power Spectral Density Method[J]. ACTA PHOTONICA SINICA, 2014, 43(5): 0535001

梁言生,姚保利,雷铭,于湘华,严绍辉,杨延龙,但旦,郜鹏,闵俊伟,叶彤. 功率谱密度法标定光镊的三维光阱刚度[J]. 光子学报, 2014, 43(5): 0535001

被引情况

【1】张玉灵,周哲海,祝连庆. 激光功率对光镊光阱刚度标定的影响. 应用光学, 2016, 37(6): 804-810

【2】曹志良,梁言生,严绍辉,周源,蔡亚楠,雷铭,李曼曼,姚保利. 不同偏振态光镊三维光阱刚度的比较研究. 光子学报, 2019, 48(7): 726002--1

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