发光学报, 2014, 35 (6): 649, 网络出版: 2014-06-10
量子点尺寸对量子点和金纳米粒子之间的荧光共振能量转移的影响
Influence of The Size of Quantum Dot on Fluorescence Resonance Energy Transfer between Quantum Dots and Au Nanoparticles
量子点 金纳米粒子 荧光共振能量转移 均相免疫检测 quantum dots Au nanoparticles fluorescence resonance energy transfer homogeneous immune-assay
摘要
量子点(QDs)所具有的一元激发多元发射的特点预示着QDs在高通量的多元量化生物信息处理领域具有应用前景。采用柠檬酸钠直接还原法制备了粒径为15 nm的Au纳米粒子, 利用静电自组装的方法构建了Au与QDs的荧光共振能量转移(FRET)系统。采用该系统研究了QDs的尺寸变化对FRET效率的影响, 获得了FRET效率和给体与受体之间交叠积分的关系。结果证明, QDs是构建多元均相免疫检测技术的一个有效给体。
Abstract
The most promising application of quantum dots (QDs) is to high throughput quantitative biological information processing because of their advantages with the different emissions for single excitation wavelength. A facile method is presented to integrate Au nanoparticles with QDs for constructing QDs@Au composite nanostructures by electrostatic interaction, in which the Au nanoparticles are synthesized with sodium citrate. Based on this composite structure, the influence of the size of QDs on the FRET efficiency is researched. These results demonstrate that the QDs are effective donors in multi-homogeneous immune-assay based on FRET.
李晓坤, 张友林, 孔祥贵. 量子点尺寸对量子点和金纳米粒子之间的荧光共振能量转移的影响[J]. 发光学报, 2014, 35(6): 649. LI Xiao-kun, ZHANG You-lin, KONG Xiang-gui. Influence of The Size of Quantum Dot on Fluorescence Resonance Energy Transfer between Quantum Dots and Au Nanoparticles[J]. Chinese Journal of Luminescence, 2014, 35(6): 649.