光子学报, 2014, 43 (9): 0930001, 网络出版: 2014-10-23
DMSO与噻酮对离体猪皮组织光学特性参量改变的定量分析
Quantitative Investigation on the Changes of Optical Property Parameters by Using DMSO and Thiazone as Enhancers for Optical Clearing of Porcine Skin in Vitro
生物医学光子学 组织光学清透 逆倍增法 光学参量 皮肤组织 噻酮 Biomedical photonics Tissue optical clearing Inverse adding-doubling method Optical parameters Skin tissue Thiazone
摘要
从定量的角度评价高渗制剂与组织光学特性参量改变的关系.利用FT-IR红外光谱仪与双积分球系统, 对离体猪皮组织在促渗剂二甲基亚砜与噻酮作用下的光谱进行测量, 并运用逆倍增方法计算得到组织的光学参量.结果表明: 离体猪皮组织的光学特性参量(吸收系数和散射系数)随着高渗制剂作用时间的增加明显减小, 光在组织中的穿透深度增加.由此可知猪皮组织的光学特性参量在两种促渗剂作用下随时间发生了动态改变, 且噻酮具有比二甲基亚砜更好的清透效果.
Abstract
The relationship between optical property parameters and the change of hyperosmotic agents were evaluated. Porcine skins, medicated without and with biocompatible hyperosmotic agents-dimethyl sulfoxide and thiazone, were measured by FT-IR spectrophotometer and double-integrating-spheres system. Optical property parameters of samples were calculated by inverse adding-doubling modeling method. The results demonstrated that with the course of the penetration of biocompatible hyperosmotic agents into porcine skin tissue, optical property parameters(absorption coefficient and scattering coefficient)of tissue samples have got decrease obviously along with the depth of light penetrating into tissue increased. In conclusion, the optical property parameters of bio-tissues could be changed by use of both agents, furthermore, thiazone has led to the better clearing than dimethyl sulfoxide.
蒋景英, 陈伟, 马聪聪, 徐可欣. DMSO与噻酮对离体猪皮组织光学特性参量改变的定量分析[J]. 光子学报, 2014, 43(9): 0930001. JIANG Jing-ying, CHEN Wei, MA Cong-cong, XU Ke-xin. Quantitative Investigation on the Changes of Optical Property Parameters by Using DMSO and Thiazone as Enhancers for Optical Clearing of Porcine Skin in Vitro[J]. ACTA PHOTONICA SINICA, 2014, 43(9): 0930001.