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基于自体荧光成像的牙菌斑量化分析

Quantitative Analysis of Dental Plaque Based on Auto-fluorescence Imaging

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摘要

本文基于牙菌斑与牙齿组织自体荧光光谱的差异性, 提出了一种利用自体荧光成像的牙菌斑量化分析方法。首先测试了牙齿样品在 405 nm激发光作用下自体荧光光谱分布, 在分析荧光颜色参量与牙菌斑含量相关性的基础上, 提出以荧光图像的 R/B作为牙菌斑含量量化的参量, 并确定使用中心波长为 490 nm的长波通滤光片对荧光光谱波段进行选择性提取。最后, 搭建实验装置获取了牙齿自体荧光图像, 利用 R/B值作为分割阈值得到了三个不同等级的牙菌斑含量与分布的量化分析结果, 与视诊结果相符。

Abstract

Based on the differences between auto-fluorescence spectra of plaque and dental tissue, a quantitative analysis method of plaque using auto-fluorescence imaging was presented. Auto-fluorescence spectrum of tooth samples excited by 405 nm was measured, on the basis of analyzing the correlation between color parameters of fluorescence and dental plaque content, R/B of fluorescence image was proposed as quantitative assessment parameter of dental plaque content, meanwhile long-wavelength pass filter with central wavelength of 490 nm was used to filter fluorescence spectral bands. Finally, experimental setup was built to obtain the auto-fluorescence image. Quantitative analysis results of three different levels of plaque content and distribution was obtained by using R/B value as segmentation thresholds, which confirmed with visual examination results.

Newport宣传-MKS新实验室计划
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中图分类号:R318.51;R781.1

DOI:10.3969/j.issn.1003-501x.2014.12.008

所属栏目:生物与医用光学

基金项目:浙江省科技厅重大科技专项 (2013C03043-5); 浙江省医药卫生一般研究计划项目 (2013KYA119); 浙江省自然基金项目 (Y2080253); 浙江省教育厅科研基金 (Y201223924); 浙江省博士后择优资助项目 (BSH1202061)

收稿日期:2014-03-04

修改稿日期:2014-07-10

网络出版日期:--

作者单位    点击查看

鞠国浩:浙江大学现代光学仪器国家重点实验室/国家光学仪器工程技术研究中心, 杭州 310027
陈庆光:杭州电子科技大学生命信息与仪器工程学院, 杭州 310018
朱海华:浙江大学医学院附属口腔医院, 浙江省口腔医院, 杭州 310027
林斌:浙江大学现代光学仪器国家重点实验室/国家光学仪器工程技术研究中心, 杭州 310027
陈晖:浙江大学医学院附属口腔医院, 浙江省口腔医院, 杭州 310027

联系人作者:鞠国浩(juguohao123@163.com)

备注:鞠国浩 (1988-), 男(汉族), 山东潍坊人。硕士研究生, 从事生物医学光子学研究。

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引用该论文

JU Guohao,CHEN Qingguang,ZHU Haihua,LIN Bin,CHEN Hui. Quantitative Analysis of Dental Plaque Based on Auto-fluorescence Imaging[J]. Opto-Electronic Engineering, 2014, 41(12): 39-45

鞠国浩,陈庆光,朱海华,林斌,陈晖. 基于自体荧光成像的牙菌斑量化分析[J]. 光电工程, 2014, 41(12): 39-45

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