针对现有微光检测系统体积大、避光效果差等不足, 对微光探测装置进行全新设计。采用反应杯与光探测头自成暗室, 减少暗室进出机构, 提高避光效果;采用斩波法扣除本底计数, 从而消除背景光引起的测量误差。性能测试结果显示:该微光探测装置的光子本底计数小于等于200光子/s, 测光上限平均值达到1.1×107光子/s, 变异系数(C)小于等于3%, 达到目前现有的微光检测系统的技术指标要求, 可用于化学发光免疫分析仪等需要进行微光信号检测的精密仪器开发, 对基于微光探测技术的仪器行业的发展具有重要意义。

The study aimed at redesigning the light detection device to compensate for deficiency of existing light detection system, such as the volume, light-protection effect and so on. It adopted the reaction cup with light detection head to form a darkroom, reduced the input and output of the darkroom, and improved the effect of light-protection. It used chopping method to deduct photon background count so as to decrease the measuring error. The performance testing results showed that the photon background count of light detection device was 200 or less photon/s. The average of photometry upper limit was 1.1×107 photon/s. Coefficient of variation (C) was 3% or less, satisfying the technical requirements of the existing light detection system. It will be available for the development of precision instruments which require light signal detection, such as chemical luminescence immunity analyzer, and will be a great significance for the development of instrument industry upon light detection technique.